Biotin anti-human MCP-1 Antibody

Pricing & Availability
Clone
5D3-F7 (See other available formats)
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
502608 50 µg 160€
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502609 500 µg 440€
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Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity*: Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MCP-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection, ICFC

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1: The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.
ELISA or ELISPOT Capture: The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.

Application References

(PubMed link indicates BioLegend citation)
  1. Peri, G., et al. 1994. J. Immunol. Meth. 174:249. (IP, IHC, WB)
  2. Rezaie-Majd, A., et al. 2002. Arterioscler Thromb Vasc Biol. 22:1194. (ICFC)
  3. Hirsch, A., et al. 1999. J. Virol. 73:404. (IP)
RRID
AB_315247 (BioLegend Cat. No. 502608)
AB_315248 (BioLegend Cat. No. 502609)

Antigen Details

Structure
CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity
Chemoattractant; regulate adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors
CCR2; CCR5; CCR10; D6; possibly L-CCR
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.

Regulation
Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID
6347 View all products for this Gene ID
UniProt
View information about CCL2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 07/02/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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