- HK1.4 (See other available formats)
- Regulatory Status
- Other Names
- Lymphocyte antigen 6 complex, locus C
- Rat IgG2c, κ
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- Product Citations
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Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- L3 cloned CTL cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.
(PubMed link indicates BioLegend citation)
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
AB_2910291 (BioLegend Cat. No. 128055)
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
- Gene ID
- 17067 View all products for this Gene ID
- View information about Ly-6C on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Pacific Blue™ anti-mouse Ly-6C
APC anti-mouse Ly-6C
Purified anti-mouse Ly-6C
Biotin anti-mouse Ly-6C
FITC anti-mouse Ly-6C
Alexa Fluor® 647 anti-mouse Ly-6C
PE anti-mouse Ly-6C
PerCP/Cyanine5.5 anti-mouse Ly-6C
PE/Cyanine7 anti-mouse Ly-6C
Alexa Fluor® 488 anti-mouse Ly-6C
Alexa Fluor® 700 anti-mouse Ly-6C
APC/Cyanine7 anti-mouse Ly-6C
PerCP anti-mouse Ly-6C
Brilliant Violet 570™ anti-mouse Ly-6C
Brilliant Violet 421™ anti-mouse Ly-6C
Brilliant Violet 510™ anti-mouse Ly-6C
Brilliant Violet 605™ anti-mouse Ly-6C
Brilliant Violet 711™ anti-mouse Ly-6C
Purified anti-mouse Ly-6C (Maxpar® Ready)
Brilliant Violet 785™ anti-mouse Ly-6C
PE/Dazzle™ 594 anti-mouse Ly-6C
APC/Fire™ 750 anti-mouse Ly-6C
TotalSeq™-A0013 anti-mouse Ly-6C
Brilliant Violet 650™ anti-mouse Ly-6C
TotalSeq™-C0013 anti-mouse Ly-6C
TotalSeq™-B0013 anti-mouse Ly-6C
APC/Fire™ 810 anti-mouse Ly-6C Antibody