- MHE-18 (See other available formats)
- Regulatory Status
- Other Names
- Immunoglobulin E
- Mouse IgG1, κ
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- Product Citations
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IgE is the seventh immunoglobulin made by B cells in the immune response.Product Details
- Antibody Type
- Host Species
- Human Ig cocktail
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
(PubMed link indicates BioLegend citation)
- Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
AB_2687222 (BioLegend Cat. No. 325515)
AB_2687223 (BioLegend Cat. No. 325516)
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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