APC anti-mouse CD34 Antibody

Pricing & Availability
Clone
MEC14.7 (See other available formats)
Other Names
Mucosialin
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
MEC14dot7_APC_110308.jpg
NIH3T3 cells stained with MEC14.7APC
  • MEC14dot7_APC_110308.jpg
    NIH3T3 cells stained with MEC14.7APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
119309 25 µg 128€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
119310 100 µg 424€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cells transfected with mouse CD34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
  2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
  3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
  4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
  5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
  7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
  8. Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.
Product Citations
  1. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  2. Mittal S, et al. 2016. Stem Cell Reports. 7:583-590. PubMed
  3. Sun J 2018. Cell stem cell. 23:355. PubMed
  4. Celik H 2018. Cancer cell. 34:741. PubMed
  5. Li J 2018. Immunity. 48:773. PubMed
Publication Library
RRID
AB_1236482 (BioLegend Cat. No. 119309)
AB_1236469 (BioLegend Cat. No. 119310)

Antigen Details

Structure
Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution
Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow
Function
Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, other selectins
Cell Type
Hematopoietic stem and progenitors, Endothelial cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account