APC anti-human Siglec-8 Antibody

Pricing & Availability
7C9 (See other available formats)
Regulatory Status
Other Names
Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Mouse IgG1, κ
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Product Citations
Human peripheral blood leukocytes were stained with CCR3 PE and Siglec-8 (clone 7C9) APC (top) or mouse IgG1, κ isotype control (bottom). Data shown was gated on the granulocyte cell population.
  • 7C9_APC_1_062612
    Human peripheral blood leukocytes were stained with CCR3 PE and Siglec-8 (clone 7C9) APC (top) or mouse IgG1, κ isotype control (bottom). Data shown was gated on the granulocyte cell population.
  • 7C9_APC_2_062612
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347105 25 tests 230€
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347106 100 tests 528€
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Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant Siglec-8 fused to human IgG Fc
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Floyd H, et al. 2000. J. Biol. Chem. 275:861.
  2. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  2. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  3. Gravastrand CS, et al. 2019. J Immunol. 203(4). PubMed
  4. Patra V, et al. 2019. Br J Dermatol. N/A. PubMed
  5. Simon SCS, et al. 2020. Oncoimmunology. 9:1727116. PubMed
  6. Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
  7. Morshed N, et al. 2020. Mol Syst Biol. 16:e9819. PubMed
  8. Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed
  9. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
AB_2561401 (BioLegend Cat. No. 347105)
AB_2561402 (BioLegend Cat. No. 347106)

Antigen Details

A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain

Eosinophils, basophils, mast cells

Cell adhesion, signal transduction
Phosphatases containing a SH2 domain
Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type
Basophils, Eosinophils, Mast cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Siglec Molecules
Antigen References

1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317.
2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608.
3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.

Gene ID
27181 View all products for this Gene ID
View information about Siglec-8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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