- Poly28410 (See other available formats)
- Regulatory Status
- Other Names
- 160 kD Neurofilament Protein, Neurofilament Triplet M Protein, Neurofilament-3 (150 Kd Medium), Neurofilament, Medium Polypeptide 150kD
Covance Catalog# PRB-575C
- Rabbit Polyclonal IgG
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- Product Citations
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Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies in the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.Product Details
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Host Species
- The immunogen was the extreme carboxyterminal region of rat NF-M, which was expressed in E. coli and purified by ion exchange chromatograpy.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
IHC-P - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:200 - 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:5000 - 1:10000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The production and characterization of this antiserum similar to monoclonal NF-M clone 3H11.
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
- Harris J, Ayyub C, Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J. Neurosci. Res. 30:47-62, 1991.
- Product Citations
AB_2565457 (BioLegend Cat. No. 841001)
- Expected MW: 145-160 kD
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 4741 View all products for this Gene ID
- View information about Neurofilament M NF-M on UniProt.org
|Anti-Neurofilament M (NF-M)||Poly28410||IHC-P, WB|
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