- SMI 35 (See other available formats)
- Regulatory Status
- Other Names
- Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Covance Catalog# SMI-35R
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Neurofilaments (NFs) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest neurofilament (NF-L) runs at 68-70 kD. The medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
IHC-P - Quality tested
WB - Verified
IHC-F, ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1:1000 - 1:2000 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1:1000 - 1:5000 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7.
Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.
Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
(PubMed link indicates BioLegend citation)
- Petzold A. 2013. J. Neuroimmunol. 262:(1-10). (ELISA)
- Lu CH, et al. 2012. PLoS One. 7:e40998. (ELISA)
- Steinacker P, et al. 2011. PLoS One. 8:e23600. (ELISA)
- Poltorak M, et al. 1993. J. Neurosci. 13:2217. (IHC-F)
- Petzold A, et al. 2011. Brain. 134:464. (WB) PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2565349 (BioLegend Cat. No. 835601)
AB_2565350 (BioLegend Cat. No. 835602)
- The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.
Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular Distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion
- Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
- Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments, Phospho-Proteins
- Antigen References
- Gene ID
- 4744 View all products for this Gene ID
- View information about Neurofilament HM NF-H NF-M Phospho on UniProt.org
|Anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated||SMI 35||IHC-P, WB, IHC-F, ELISA|
|Purified anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated||SMI 35||IHC-P, WB, IHC-F|
|Biotin anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated||SMI 35||WB|
|Alexa Fluor® 594 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated||SMI 35||IHC-P, ICC|
|HRP anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated||SMI 35||IHC-P, WB|
|Alexa Fluor® 647 anti NF-H/NF-M, Hypophosphorylated||SMI 35||IHC-P|
|Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated||SMI 35||IHC-P, SB|
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated
Purified anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated
Biotin anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated
Alexa Fluor® 594 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated
HRP anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated
Alexa Fluor® 647 anti NF-H/NF-M, Hypophosphorylated
Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated