- Clone
- Poly19246 (See other available formats)
- Other Names
- Mitotic Spindle Assembly Checkpoint Protein Mad2A
- Previously
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Covance Catalog# PRB-452C
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-MAD2 Antibody, clone Poly19246, at 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 924601 | 200 µl | 552€ | ||||
Mitotic arrest deficient protein 2 (MAD2) is a 24kD kinetochore-associated protein which plays an important role in the orderly progression of cell division.
Product DetailsProduct Details
- Reactivity
- Human, Mouse
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against a recombinant full length human MAD2 protein.
- Preparation
- Serum
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500 - 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone is not recommended for immunocytochemistry or immunohistochemistry.
*Predicted MW = 24-27 kD
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Y Li, R Benezra. 1996. Science. 274:246.
- K Wassmann, R Benezra. 1998. Proc. Natl. Acad. Sci. U.S.A. 95:11193.
- LS Michel, et al. 2001. Nature. 409:355.
- N den Elzen, J Pines. 2001. J. Cell. Biol. 153:121.
- RH Chen, et al. 1996. Science. 274:242.
- Product Citations
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- RRID
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AB_2565454 (BioLegend Cat. No. 924601)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 4085 View all products for this Gene ID
- UniProt
- View information about MAD2 on UniProt.org
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