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Anti-MAD2 Antibody (Previously Covance catalog# PRB-452C)

Pricing & Availability
Clone
Poly19246 (See other available formats)
Other Names
Mitotic Spindle Assembly Checkpoint Protein Mad2A
Previously
Covance Catalog# PRB-452C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly19246_MAD2_Antibody_WB_032119
Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-MAD2 Antibody, clone Poly19246, at 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • Poly19246_MAD2_Antibody_WB_032119
    Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-MAD2 Antibody, clone Poly19246, at 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
924601 200 µL 552€
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Description

Mitotic arrest deficient protein 2 (MAD2) is a 24kD kinetochore-associated protein which plays an important role in the orderly progression of cell division.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against a recombinant full length human MAD2 protein.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500 - 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for immunocytochemistry or immunohistochemistry.

 

*Predicted MW = 24-27 kD

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Y Li, R Benezra. 1996. Science. 274:246.
  2. K Wassmann, R Benezra. 1998. Proc. Natl. Acad. Sci. U.S.A. 95:11193.
  3. LS Michel, et al. 2001. Nature. 409:355.
  4. N den Elzen, J Pines. 2001. J. Cell. Biol. 153:121.
  5. RH Chen, et al. 1996. Science. 274:242.
Product Citations
  1. Bärenz F, et al. 2018. Mol Biol Cell. 29:3063. PubMed
  2. Vázquez‐Diez C et al. 2019. Current biology : CB. 29(5):865-873 . PubMed
  3. Paim LMG, et al. 2019. Nat Commun. 3.773611111. PubMed
  4. Conti D, et al. 2019. Elife. 8:e49325. PubMed
RRID
AB_2565454 (BioLegend Cat. No. 924601)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
4085 View all products for this Gene ID
UniProt
View information about MAD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09/13/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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