Alexa Fluor® 647 anti-SPI1 (PU.1) Antibody

Pricing & Availability
Clone
7C6B05 (See other available formats)
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Transcription factor PU.1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7C6B05_AF647_SIP1_Antibody_1_ICFC_082115
Human peripheral blood lymphocytes were surface stained with CD19 Brilliant Violet 421™ and then were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-human SPI1 (clone 7C6B05) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 7C6B05_AF647_SIP1_Antibody_1_ICFC_082115
    Human peripheral blood lymphocytes were surface stained with CD19 Brilliant Violet 421™ and then were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-human SPI1 (clone 7C6B05) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 7C6B05_AF647_SIP1_Antibody_2_ICFC_082115
  • 7C6B05_AF647_SIP1_Antibody_3_ICC_070918
    Human peripheral blood monocyte-derived macrophages were fixed with 1% paraformaldehyde (PFA) and permeabilized with 0.5% TX-100. Then the cells were stained with 5 µg/ml of SPI1 (clone 7C6B05) Alexa Fluor® 647 (red) overnight at 4°C and followed by 5 µg/ml of CD11b Alexa Fluor® 488 (green) staining for 30 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 60X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
658003 25 tests 240€
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658008 100 µg 520€
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658004 100 tests 520€
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Need larger quantities of this item?
Request Bulk Quote
Description

SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death.  Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SPI1 recombinant protein
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining using µsize, the suggested use of this reagent is 0.5 µg per million cells in 100 µl volume. For flow cytometric staining using tests sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunocytochemistry, a concentration range of 2.5 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Dutertre CA et al. 2019. Immunity. 51(3):573-589 . PubMed
RRID
AB_2563262 (BioLegend Cat. No. 658003)
AB_2563895 (BioLegend Cat. No. 658008)
AB_2563263 (BioLegend Cat. No. 658004)

Antigen Details

Structure
270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
Distribution

Nucleus.

Function
SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmelé P, et al. 2010. Cancer Res. 70:6757.

Gene ID
6688 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12/22/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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