Alexa Fluor® 647 anti-mouse FOXP3 Antibody

Pricing & Availability
Clone
MF-14 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Rat IgG2b, κ
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Product Citations
publications
MF-14_AF647_FOXP3_Antibody_ICFC_1_042015
C57BL/6 splenocytes were surface stained with CD4 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone MF-14) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • MF-14_AF647_FOXP3_Antibody_ICFC_1_042015
    C57BL/6 splenocytes were surface stained with CD4 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone MF-14) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • MF-14_AF647_FOXP3_Antibody_ICFC_2_042015
Compare all formats See Alexa Fluor® 647 spectral data
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126407 25 µg 302€
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126408 100 µg 592€
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Description

FOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Ono M, et al. 2007. Nature 446:685.
  2. Hori S, et al. 2003. Science 299:1057.
  3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
  4. Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
  5. Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
  6. Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
Product Citations
  1. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  2. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  3. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  4. Leier A, et al. 2022. Mol Ther Nucleic Acids. 28:261. PubMed
  5. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  6. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  7. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  8. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  9. Feng H, et al. 2020. J Dermatol. 47:147. PubMed
  10. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  11. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  12. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  13. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  14. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  15. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  16. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  17. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  18. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  19. Zhang X, et al. 2021. Front Pharmacol. 12:629513. PubMed
  20. Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed
  21. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  22. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  23. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  24. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  25. Molgora M, et al. 2020. Cell. 182:886. PubMed
  26. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  27. Sheng J, et al. 2021. eLife. 10:00. PubMed
  28. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  29. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  30. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  31. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  32. Gray CC, et al. 2022. Front Immunol. 13:861670. PubMed
  33. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  34. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID
AB_1089116 (BioLegend Cat. No. 126407)
AB_1089115 (BioLegend Cat. No. 126408)

Antigen Details

Structure
50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
Distribution

Nuclear; expressed in Treg cells.

Function
Master regulatory gene in Treg cell development, crucial for immune homeostasis.
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.

Regulation
Present at high level in T reg cells. Induced by T cell activation.
Gene ID
20371 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 4    Revision Date: 04/21/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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