Alexa Fluor® 647 anti-Lin28A Antibody

Pricing & Availability
Clone
14E6-4E6 (See other available formats)
Regulatory Status
RUO
Other Names
Protein Lin-28 Homolog A, Lin-28A, CSDD1, ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
a. 14E6-4E6_A647_Lin28A_2_Antibody_031722
NTERA2 cells (positive target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-LIN28A (clone 14E6-4E6) (filled histogram) or Alexa Fluor® 647 Mouse IgG2a, κ isotype control (Cat. No. 400240) (open histogram) followed by PE goat anti-mouse IgG (Cat. No. 405307).
  • a. 14E6-4E6_A647_Lin28A_2_Antibody_031722
    NTERA2 cells (positive target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-LIN28A (clone 14E6-4E6) (filled histogram) or Alexa Fluor® 647 Mouse IgG2a, κ isotype control (Cat. No. 400240) (open histogram) followed by PE goat anti-mouse IgG (Cat. No. 405307).
  • b. 14E6-4E6_A647_Lin28A_1_Antibody_031722
    HeLa cells (negative target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-LIN28A (clone 14E6-4E6), (filled histogram) or Alexa Fluor® 647 Mouse IgG2a, κ isotype control (Cat. No. 400240) (open histogram).
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657805 25 tests 294€
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Description

LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Lin28A from E. coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is specific for Lin28A does not recognize Lin28B.

RRID
AB_2924622 (BioLegend Cat. No. 657805)

Antigen Details

Structure
209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
Distribution

Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules

Function
Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
Interaction
EIF3S2, NCL and ZCCHC11/TUT4
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
Antigen References

1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395.
2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474.
3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483.
4. Cho J, et al. 2012. Cell. 151:765.
5. Li N, et al. 2012. J. Biol. Chem. 287:17386.
6. Zhu H, et al. 2011. Cell 147:81.

Gene ID
79727 View all products for this Gene ID
UniProt
View information about Lin28A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/17/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Lin28A

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