- GHI/61 (See other available formats)
- Regulatory Status
- VI M38
- Other Names
- GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.Product Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
SB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
(PubMed link indicates BioLegend citation)
- Pulford K, et al. 1992. Immunology 75:588. (ICC, IP, WB)
- Law SK, et al. 1993. Eur. J. Immunol. 23:2320.
- Madsen M, et al. 2004. J. Biol. Chem. 279:51561.
- Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
- Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2563474 (BioLegend Cat. No. 333619)
AB_2563475 (BioLegend Cat. No. 333620)
- 134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
- Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
- Haptoglobin-hemoglobin complex, TWEAK
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179
- Gene ID
- 9332 View all products for this Gene ID
- View information about CD163 on UniProt.org
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
|PerCP anti-human CD163||GHI/61||FC|
|Purified anti-human CD163||GHI/61||FC, ICC, IP, WB|
|Biotin anti-human CD163||GHI/61||FC|
|PE anti-human CD163||GHI/61||FC|
|PerCP/Cyanine5.5 anti-human CD163||GHI/61||FC|
|APC anti-human CD163||GHI/61||FC|
|Brilliant Violet 421™ anti-human CD163||GHI/61||FC|
|PE/Cyanine7 anti-human CD163||GHI/61||FC|
|Brilliant Violet 605™ anti-human CD163||GHI/61||FC|
|FITC anti-human CD163||GHI/61||FC|
|Alexa Fluor® 647 anti-human CD163||GHI/61||FC, SB|
|APC/Cyanine7 anti-human CD163||GHI/61||FC|
|PE/Dazzle™ 594 anti-human CD163||GHI/61||FC|
|Brilliant Violet 510™ anti-human CD163||GHI/61||FC|
|Brilliant Violet 711™ anti-human CD163||GHI/61||FC|
|Brilliant Violet 785™ anti-human CD163||GHI/61||FC|
|APC/Fire™ 750 anti-human CD163||GHI/61||FC|
|TotalSeq™-A0358 anti-human CD163||GHI/61||PG|
|TotalSeq™-C0358 anti-human CD163||GHI/61||PG|
|TotalSeq™-B0358 anti-human CD163||GHI/61||PG|
|PE/Cyanine5 anti-human CD163||GHI/61||FC|
|TotalSeq™-D0358 anti-human CD163||GHI/61||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
PerCP anti-human CD163
Purified anti-human CD163
Biotin anti-human CD163
PE anti-human CD163
PerCP/Cyanine5.5 anti-human CD163
APC anti-human CD163
Brilliant Violet 421™ anti-human CD163
PE/Cyanine7 anti-human CD163
Brilliant Violet 605™ anti-human CD163
FITC anti-human CD163
Alexa Fluor® 647 anti-human CD163
APC/Cyanine7 anti-human CD163
PE/Dazzle™ 594 anti-human CD163
Brilliant Violet 510™ anti-human CD163
Brilliant Violet 711™ anti-human CD163
Brilliant Violet 785™ anti-human CD163
APC/Fire™ 750 anti-human CD163
TotalSeq™-A0358 anti-human CD163
TotalSeq™-C0358 anti-human CD163
TotalSeq™-B0358 anti-human CD163
PE/Cyanine5 anti-human CD163
TotalSeq™-D0358 anti-human CD163