Alexa Fluor® 647 anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_A647_DYKDDDDK_Tag_Antibody_1_092817
HeLa cells transiently transfected with DYKDDDDK tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 647 anti-DYKDDDDK Tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective.
  • L5_A647_DYKDDDDK_Tag_Antibody_1_092817
    HeLa cells transiently transfected with DYKDDDDK tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 647 anti-DYKDDDDK Tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective.
  • L5_A647_DYKDDDDK_Tag_Antibody_2_092817
    HeLa cells transiently transfected with DYKDDDDK tag fused protein or non-transfected were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 (2 µg/ml), 1:1000 (0.5 µg/ml) and 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 647 anti-DYKDDDDK Tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective using KEYENCE BZ-X700 fluorescence microscope.
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Cat # Size Price Quantity Avail. Save
637315 25 µg 216€
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
DYKDDDDK tag epitope
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1:250-1:2500 (0.2 - 2 µg/mL) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
RRID
AB_2716154 (BioLegend Cat. No. 637315)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08/07/2019

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