Alexa Fluor® 647 anti-Cytochrome c Antibody

Pricing & Availability
Clone
6H2.B4 (See other available formats)
Other Names
Cyt c
Isotype
Mouse IgG1, κ
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Product Citations
publications
6H2.B4_AF647_Cytochrome_C_Antibody_ICFC_122414
C57BL/6 splenocytes were treated with BioLegend's Fixation Buffer and Permeabilization Wash Buffer, and then were stained with Cytochrome C (clone 6H2-B4) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • 6H2.B4_AF647_Cytochrome_C_Antibody_ICFC_122414
    C57BL/6 splenocytes were treated with BioLegend's Fixation Buffer and Permeabilization Wash Buffer, and then were stained with Cytochrome C (clone 6H2-B4) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
612310 100 µg 360€
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Description

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat cyt c-OVA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
  2. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
  3. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
  4. Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
  5. Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
  6. She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
  7. McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
Product Citations
  1. Jenson J, et al. 2017. Elife. 10.7554/eLife.25541. PubMed
  2. Ramsey HE, et al. 2018. Cancer Discov. 8:1566. PubMed
Publication Library
RRID
AB_2565241 (BioLegend Cat. No. 612310)

Antigen Details

Structure
Heme binding domain; 15 kD
Distribution

Mitochondrial intermembrane space

Function
Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction
Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References

1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.

Gene ID
1355 View all products for this Gene ID
UniProt
View information about Cytochrome c on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/02/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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