Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_G88_AF594_CD326_Antibody_IHCF_092917
C57BL/6 mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml CD90.2 (clone 30-H12) Alexa Fluor® 488 (green) and CD326 (clone G8.8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 1_G88_AF594_CD326_Antibody_IHCF_092917
    C57BL/6 mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml CD90.2 (clone 30-H12) Alexa Fluor® 488 (green) and CD326 (clone G8.8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 2_G88_AF594_CD326_Antibody_IHCF_092917
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of CD326 (clone G8.8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
  • 3_G88_AF594_CD326_Antibody_IF_092917
    TE-71 thymic epithelial mouse cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the cells was stained with 1 µg/ml of CD326 (clone G8.8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
  • 4_G8.8_AF594_CD326_Antibody_IHCF_3_121018
    Dissected C57/B6 mouse small intestine was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 2.5 µg/mL of anti-mouse Tubulin Beta 3 (clone AA10) Alexa Fluor® 647 (green) and 2.5 µg/mL of anti-mouse CD326 (clone G8.8) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 5_Still-image_0326-3_20X-1_3
    Formalin-fixed, 300 micron-thick mouse large intestine section was blocked, permeabilized and stained overnight with Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 647 (green) at 2.5 µg/mL, Helix NP™ Green (blue), and CD326 (EpCAM)(clone G8.8) Alexa Fluor® 594 (magenta) at 5 µg/mL, optically cleared, and analyzed at 205 μm imaging depth on a confocal microscope. Watch the video.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
118222 100 µg 392€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F, 3-D IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.0 - 5.0 μg/m. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
RRID
AB_2563322 (BioLegend Cat. No. 118222)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/23/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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