- Clone
- PMab-1 (See other available formats)
- Other Names
- T1a, gp38, stromal cell marker
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 488 (open histogram). -
C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10µg/ml of Alexa Fluor® 488 anti-mouse podoplanin (clone PMab-1, green), 10 µg/ml of Alexa Fluor® 594 anti-mouse CD326 (clone G8.8, red) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 156207 | 25 µg | 240€ | ||||
| 156208 | 100 µg | 504€ | ||||
The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Peptide (38-51 amino acids of mouse podoplanin)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
- RRID
-
AB_2814079 (BioLegend Cat. No. 156207)
AB_2814080 (BioLegend Cat. No. 156208)
Antigen Details
- Structure
- 43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.
- Distribution
-
Expressed on epithelial and mesothelial cells.
- Function
- Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
- Interaction
- Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
- Ligand/Receptor
- CLEC1B
- Cell Type
- Epithelial cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Farr A, et al. 1992. J Histochem Cytochem. 40:651.
- Schacht V, et al. 2005. Am J Pathol. 166:913.
- Gene ID
- 14726 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.