Flow cytometry is a useful application used in countless labs for the phenotyping of immune cells. When discussing flow cytometers, fluorophore development is inextricably intertwined. As more and more fluorophores were generated, more complex cytometers were invented to increase multicolor flow cytometry options. Decades ago, multicolor flow experiments were capped at 2 or 3 colors; as cytometers advance this number has grown to >20 colors and continues to grow. 

Take a look at our timeline to learn how the flow cytometers and your favorite fluorophores came about. To navigate through the presentation use the forward (>) and backwards (<) arrows. Click on the rectangle in the bottom right to view in full screen.

References:
1. Alivisatos, A.P. 1996. Science. 271:933. JSTOR
2. Bendall, S.C. et al. 2012. Trends Immunol. 33:323. Pubmed
3. Panchuk-Voloshina, N. et al. 1999. J. Histochem. Cytochem. 47:1179. Pubmed
4. Shapiro, H. Practical Flow Cytometry, 4th ed. Hoboken: John Wiley & Sons, Inc. 2003. Wiley
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