Direct-Blot™ antibodies are primary antibodies directly conjugated to horseradish peroxidase (HRP), eliminating the need for a secondary antibody. Direct-Blot™ antibodies are able to help you save time and money by eliminating a number of steps and reagents from your normal western blot workflow. This will help you to reduce the cost and to get your experiment completed quicker. Other great benefits of Direct-Blot™ HRP include a shorter protocols, ease of use in multiplexing, and the ability to combine with in Immunoprecipitation assays. In addition, these reagents are highly sensitive and have excellent stability.

 

You can learn more by taking a look at our Direct-Blot™ western blotting protocol and webpage. You can also view our protocol video below to see how it compares to traditional western blotting and learn how it can save you time and money.

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Direct-Blot™ HRP anti-β-Actin Antibody

Whole cell lysates (15 µg protein) from HeLa cultures were resolved by electrophoresis (4-20% Trisglycine gel), transferred to nitrocellulose, and probed with 1:10,000, 1:40,000, and 1:100,000 dilutions of Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A, or a 1:10,000 dilution of purified anti-β-actin Antibody, clone W16197A (upper). Proteins were visualized using a 1:3000 diluted HRP anti rat-IgG secondary antibody for purifi ed anti-β-actin Antibody and chemiluminescence detection. Ponceau S staining was used as loading control (lower). Lane M: MW ladder.