Tutorial: See 3D (Ce3D™): Enhanced Tissue Clearing for Multicolor Microscopy


Biological tissues are generally composed of proteins, lipids, and water, each of which has a different refractive index (RI). Differences in refractive indices, or RI mismatch, cause the scattering of light in the tissue and result in tissue opacity. Tissue clearing reagents aim to reduce light scattering by normalizing the RI throughout the tissue, thus making the tissue transparent. Optical clearing is a valuable application that allows us gain a better understating of spatial composition, phenotypic and subtype identity, and cellular networks. Our Ce3D™ Tissue Clearing Solution pairs perfectly with our expansive portfolio of antibodies, generating mesmerizing 3D images with unparalleled depth.

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