Recombinant Human IL-6 (carrier-free)

Product Details

Source

Human IL-6, amino acids Pro29-Met212 (Accession # NM_000600) was expressed in E. coli.

Molecular Mass

The 185 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,041 Da. The DTT-reduced and non-reduced protein migrate at approximately 23kDa by SDS-PAGE.

Purity

Purity is >98%, as determined by Coomassie stained SDS-PAGE.

Formulation

PBS, pH 7.2, 0.5M NaCl, 10% Glycerol

Endotoxin Level

Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.

Concentration

10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

The ED₅₀ is 4-20 pg/ml, corresponding to a specific activity of 0.5-2.5 X10⁸ units/mg, as determined by a dose dependent stimulation in a 7TD1 cell proliferation assay.

The specific activity of recombinant human IL-6 is approximately 5.0 x 10⁴ IU/µg when compared against the 1st WHO International Standard for Interleukin-6 (NIBSC code: 89/548) as determined by the dose dependent stimulation of 7TD1 cell proliferation.

For more information on specific activity, please visit the Recombinant Protein Unit Conversions page.

Application

Bioassay

Recommended Usage

Use when high specific biological activity is required.

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

1. Reitsma JM, et al. 2013. J Virol. 87:10763. PubMed

Product Citations

1. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
2. Kaiser R, et al. 2021. JCI Insight. 6:e150862. PubMed
3. Reitsma J, et al. 2013. J Virol. 87:10763. PubMed
4. Zhang H, et al. 2020. Front Cell Dev Biol. 8:205. PubMed
5. Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
6. Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed
7. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
8. Fulciniti M, et al. 2018. Cell Rep. 25:3693. PubMed
9. Yamaguchi Y, et al. 2022. J Immunother Cancer. 10:. PubMed
10. Ibrahim ML, et al. 2018. Cell Rep. 25:3036. PubMed
11. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
12. Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
13. Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed
14. Wong GK, et al. 2019. JCI Insight. 4. PubMed
15. Ianniciello A, et al. 2021. Sci Transl Med. 13:eabd5016. PubMed
16. Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed
17. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed

Antigen Details

Structure

Neuropoietic cytokine family

Distribution

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Function

Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β

Interaction

T cells, B cells, hepatocytes, cholinergic neurons

Ligand/Receptor

Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)

Bioactivity

Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction

Cell Type

Hematopoietic stem and progenitors

Biology Area

Cell Biology, Immunology, Innate Immunity, Stem Cells

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Gene ID

3569 View all products for this Gene ID

UniProt

View information about IL-6 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Certificate of Analysis