Purified anti-Caspase-1 Antibody

Pricing & Availability
Clone
5B10 (See other available formats)
Other Names
ICE
Isotype
Rat IgG2a
Ave. Rating
Submit a Review
Product Citations
publications
5B10_Purified_Caspase-1_Antibody_WB_101716
Total cell lysates (15 µg protein) from Jurkat and P338D1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Caspase 1 antibody (clone 5B10). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. GAPDH Antibody was used as a loading control.
  • 5B10_Purified_Caspase-1_Antibody_WB_101716
    Total cell lysates (15 µg protein) from Jurkat and P338D1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Caspase 1 antibody (clone 5B10). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. GAPDH Antibody was used as a loading control.
Cat # Size Price Quantity Avail. Save
645101 25 µg 84€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
645102 100 µg 180€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Caspase -1 plays a vital role in cleaving the inactive procytokine pro-IL-1 beta to generate the active 17 kd mature form of IL-1beta, a key mediator of inflammation. Active caspase-1 (ICE) is derived a single proenzyme of 45 Kd. An active ICE comprises two subunits of P20 and two subunits of P10 after removal a large amino-terminal pro-domain and a small linker peptide. Caspase-1 is reported to be involved in CD95- mediated apoptosis. Four alternatively spliced variants of caspase-1 have been reported, with mw ranging from 30-48 kd.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Bacterial fusion protein (p20)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1-2µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Kang SJ, et al. 2000. J Cell Biol 149:613
  2. Kang SJ, et al. 2002. Cell Death and Differentiation. 9:1115
Product Citations
  1. Zhu Q, et al. 2016. Am J Physiol Renal Physiol. 310: F621-F627. PubMed
  2. Liu L, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjw219. PubMed
RRID
AB_2068901 (BioLegend Cat. No. 645101)
AB_2068900 (BioLegend Cat. No. 645102)

Antigen Details

Structure
The whole length of Caspase-1 is a 404 amino acid polypeptide chain with MW of 45kd. The active Caspase -1 is a tetramer compariseing two subunits of 20Kd and 10Kd.
Distribution

Predominantly in the cytoplasm, some localized to the external cell membrane.

Function
Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta.
Ligand/Receptor
Pro-IL1 beta
Bioactivity
IL-1beta converting enzyme (ICE)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Wilson KP et al. 1994. Nature 370:251
2. Cohen GM:1997 Biochem J 326:1

Gene ID
834 View all products for this Gene ID
UniProt
View information about Caspase-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/17/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account