Ultra-LEAF™ Purified anti-SARS-CoV-2 S Protein RBD Antibody

Pricing & Availability
Clone
A20100I (See other available formats)
Regulatory Status
RUO
Other Names
S1 RBD, Spike Protein RBD, S1 Receptor-binding Domain, Spike Receptor-binding Domain
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A20100I_ULEAF_SARS-CoV-2-S_Antibody_020221.png
Recombinant human ACE2 (Cat. No. 792002) (black circles) binds to immobilized recombinant SARS-CoV-2 S protein RBD-Fc chimera (Cat. No. 793104) in a dose-dependent manner. Purified anti-SARS-CoV-2 S protein RBD antibody (clone A20100I) (purple squares) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.5 µg/mL recombinant human ACE2 to 1.0 µg/mL immobilized recombinant SARS-CoV-2 S protein RBD-Fc chimera. ND50 range: 0.05 - 0.25 µg/mL.
  • A20100I_ULEAF_SARS-CoV-2-S_Antibody_020221.png
    Recombinant human ACE2 (Cat. No. 792002) (black circles) binds to immobilized recombinant SARS-CoV-2 S protein RBD-Fc chimera (Cat. No. 793104) in a dose-dependent manner. Purified anti-SARS-CoV-2 S protein RBD antibody (clone A20100I) (purple squares) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.5 µg/mL recombinant human ACE2 to 1.0 µg/mL immobilized recombinant SARS-CoV-2 S protein RBD-Fc chimera. ND50 range: 0.05 - 0.25 µg/mL.
Cat # Size Price Quantity Check Availability Save
944803 100 µg 287€
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944804 1 mg 964€
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Description

SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The corona virus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit under goes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SARS-CoV-2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL recombinant human ACE2 (Cat. No. 792002) to 1 µg/mL immobilized recombinant SARS-CoV-2 S protein RBD-Fc chimera (Cat. No. 793104). ND50 range: 0.05 - 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892509 (BioLegend Cat. No. 944803)
AB_2892509 (BioLegend Cat. No. 944804)

Antigen Details

Structure
Disulfide linked homodimer
Distribution

SARS-CoV-2

Function
Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection
Interaction
Lung cells
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References

1. Lu R, et al. 2020. Lancet. 395:565-574. 
2. Li F. 2016. Annu Rev Virol. 29:237-261. 
3. Belouzard S, et al. 2012. Viruses. 4:1011-33. 
4. Song W, et al. 2018. PLoS Pathog. 14(8). 
5. Li F, et al. 2020. Nature. 581:221-224.

Gene ID
NA
UniProt
View information about Viral Protein on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02/02/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-SARS-CoV-2 S Protein RBD

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