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Ultra-LEAF™ Purified anti-mouse CXCL10 (IP-10) Antibody

Pricing & Availability
Clone
A16079E (See other available formats)
Regulatory Status
RUO
Other Names
Interferon gamma-inducible protein 10 (IP-10), Interferon gamma induced factor, gIP-10, IFI10, INP10, cytokine responsive gene-2 (CRG2)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A16079E_ULEAF_CXCL10_Antibody_030722
Recombinant mouse CXCL10 (IP-10) (Cat. No. 573604) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectants. Ultra-LEAF™ purified anti-mouse CXCL10 (IP-10) (clone A16079E) (purple squares) neutralizes recombinant mouse CXCL10 (IP-10) induced chemotaxis at 8 ng/mL on mouse Baf3-mCXCR3 cells in a dose-dependent manner, whereas GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.6 - 3 µg/mL
  • A16079E_ULEAF_CXCL10_Antibody_030722
    Recombinant mouse CXCL10 (IP-10) (Cat. No. 573604) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectants. Ultra-LEAF™ purified anti-mouse CXCL10 (IP-10) (clone A16079E) (purple squares) neutralizes recombinant mouse CXCL10 (IP-10) induced chemotaxis at 8 ng/mL on mouse Baf3-mCXCR3 cells in a dose-dependent manner, whereas GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.6 - 3 µg/mL
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604453 100 µg 287€
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604454 1 mg 748€
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Description

CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11. CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ. CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many inflammatory diseases, including arthritis, type I diabetes, experimental autoimmune encepahlomyelitis, atherosclerosis, allograft rejection, and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CXCL10 (IP-10)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant mouse CXCL10 (IP-10) (Cat. No. 573604) at 8 ng/mL on mouse Baf3- mCXCR3 transfectants. ND50 range: 0.6 - 3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922639 (BioLegend Cat. No. 604453)
AB_2922639 (BioLegend Cat. No. 604454)

Antigen Details

Structure
Chemokine
Distribution

Interferon gamma-stimulated keratinocytes, monocytes, macrophages, fibroblasts, endothelial, and T cells

Function
CXCL10 chemoattracts CD4, Th1, CD8, and NK and NKT cells. CXCL10 is induced by inflammatory cytokines. NH2-terminal cleavage of CXCL10 by DPP-IV/CD26 impairs its chemoattracting capacity and CXCR3 signaling ability.
Interaction
Activated T cells, Th1 cells, regulatory T cells, NK cells, NKT cells, endothelial cells, and fibroblasts
Ligand/Receptor
CXCR3
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Luster AD, et al. 1985. Nature 315:672-6.
  2. Vanguri P, et al. 1990. J. Biol. Chem. 265:15049-57.
  3. Loos T, et al. 2008. Blood 112:2648-56.
  4. Crawford MA, et al. 2009. Infect. Immun. 77:1664-78.
  5. Campanella GS, et al. 2010. PLoS One 5:e12700.
  6. Hoerning A. 2011. Eur. J. Immunol. 41:2291-302.
Gene ID
15945 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/07/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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