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Ultra-LEAF™ Purified anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
W19361A (See other available formats)
Regulatory Status
RUO
Other Names
Mast/stem cell growth factor receptor Kit, SCFR, c-Kit, Kit, CD117
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19361A_ULEAF_CD117_C-Kit_Antibody_101821
Recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806) (black circles) binds to immobilized recombinant mouse SCF (Cat. No. 579706). Ultra-LEAF™ purified anti-mouse CD117 (clone W19361A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 1 µg/mL recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806) to 2 µg/mL immobilized recombinant mouse SCF (Cat. No. 579706). ND50 range: 0.5 - 2 µg/mL.
  • W19361A_ULEAF_CD117_C-Kit_Antibody_101821
    Recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806) (black circles) binds to immobilized recombinant mouse SCF (Cat. No. 579706). Ultra-LEAF™ purified anti-mouse CD117 (clone W19361A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 1 µg/mL recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806) to 2 µg/mL immobilized recombinant mouse SCF (Cat. No. 579706). ND50 range: 0.5 - 2 µg/mL.
Cat # Size Price Quantity Check Availability Save
946803 100 µg 137€
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946804 1 mg 524€
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit and stem cell factor receptor (SCFR). It belongs to the CSF-1/PDGF receptor subfamily. CD117 is a receptor for stem cell factor (SCF) or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells, and acute myeloid leukemia cells (AML). c-Kit ligand binding of CD117 induces dimerization and phosphorylation of CD117, and activates the downstream signaling, including PI3K, JAK/STAT, PLC-γ and MAPK pathways. Consequently, it stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The turnover rate of CD117 depends on ligand binding and its kinase activity. CD117 is also downregulated by PKC-mediated proteolytic cleavage. Additionlly, ADAM-17 mediates shedding of CD117 on the surface of mast cells. SCF/CD117 ligation protects vascular smooth musle cells against apoptosis and increases hyperplasia after vascular injury. The ligation also regulates cell survival and cell infiltration of mast cells in tumor microenviroment. Some evidence indicates that CD117 constitutively associates with interleukin-1 receptor accessory protein (IL-1RAcP), and activates IL-33-induced cytokine production upon costimulation with c-Kit ligand in primary mast cells. CD117 is upregulated on dendritic cells by house dust mite allergen and mucosal adjuvant cholera toxin (CT), and consequently regulates Th2's and Th17's responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD117 (c-Kit)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 1 µg/mL recombinant mouse CD117 (c-Kit)-Fc chimera (Cat. No. 790806) to 2 µg/mL immobilized recombinant mouse SCF (Cat. No. 579706). ND50 range: 0.5 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922647 (BioLegend Cat. No. 946803)
AB_2922647 (BioLegend Cat. No. 946804)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Interaction
Endothelial cells, hematopoietic stem and progenitors, mast cells
Ligand/Receptor
Stem cell factor (SCF)
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Nocka K, et al. 1990. EMBO J. 9:3287-94.
  2. Reith AD, et al. 1991. EMBO J. 9:2451-9.
  3. Cardoso HJ, et al. 2017. J Cell Commun Signal. 4:297-307.
  4. Sette C, et al. 1998. J Cell Biol. 142:1063-74.
  5. Kurosawa K, et al. 1996. Blood. 87:2235-43.
  6. Drube S, et al. 2010. Blood. 115:3899-906.
  7. Lemmon MA, et al. 1997. J Biol Chem. 272:6311-7.
  8. Yee NS, et al. 1994. J Biol Chem. 269:31991-8.
  9. Cruz AC, et al. 2004. J Biol Chem. 279:5612-20.
  10. Kimura Y, et al. 2011. PLoS One. 6:e26918.
  11. Krishnamoorthy N, et al. 2008. Nat Med. 14:565-73.
  12. Huang B, et al. 2008. Blood. 112:1269-79.
Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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