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Ultra-LEAF™ Purified anti-human PLAUR (uPAR) Antibody

Pricing & Availability
Clone
A20095H (See other available formats)
Regulatory Status
RUO
Other Names
CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
A20095H_ULEAF_PLAUR_Antibody_081621
Biotinylated recombinant human PLAUR (uPAR) (black circles) binds to immobilized recombinant human u-Plasminogen Activator (Urokinase) (Cat. No. 755304). Ultra-LEAF™ purified anti-human PLAUR (uPAR) antibody (clone A20095H) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified mouse IgM, κ isotype ctrl antibody (Cat. No. 401630) (gray triangles) does not have the effect. This antibody blocks the binding of 3 µg/mL biotinylated recombinant human PLAUR (uPAR) to 2 µg/mL immobilized recombinant human u-Plasminogen Activator (Urokinase). ND50 range: 4 - 20 µg/mL.
  • A20095H_ULEAF_PLAUR_Antibody_081621
    Biotinylated recombinant human PLAUR (uPAR) (black circles) binds to immobilized recombinant human u-Plasminogen Activator (Urokinase) (Cat. No. 755304). Ultra-LEAF™ purified anti-human PLAUR (uPAR) antibody (clone A20095H) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified mouse IgM, κ isotype ctrl antibody (Cat. No. 401630) (gray triangles) does not have the effect. This antibody blocks the binding of 3 µg/mL biotinylated recombinant human PLAUR (uPAR) to 2 µg/mL immobilized recombinant human u-Plasminogen Activator (Urokinase). ND50 range: 4 - 20 µg/mL.
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946203 100 µg 287€
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946204 1 mg 964€
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Description

PLAUR (plasminogen activator urokinase receptor), also known as urokinase plasminogen activator-receptor (uPAR), is part of the plasminogen activation system, which is involved in tissue reorganization, cell migration, cell cycle regulation, and cell adhesion. PLAUR was originally identified as a saturable binding site for urokinase on the cell surface, and thus, restricting plasminogen activation to the immediate vicinity of the cell membrane. Due to lack of transmembrane or cytoplasmic domains, PLAUR may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its C-terminus. A soluble form of PLAUR has been identified. The membrane-bound PLAUR is expressed on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, and keratinocytes. This molecule plays a role in cell adhesion and chemotaxis through binding to β1, β2, and β3 integrins. Recently, it was found that soluble PLAUR is increased in plasma of patients with active lupus nephritis, suggesting an association with pathological features of the disease. In addition, soluble PLAUR has been linked as a causative factor and also a biomarker of several diseases including tumor metastasis, focal and segmental glomerulosclerosis, hepatocellular carcinoma, and coronary artery disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human PLAUR (uPAR)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 3 µg/mL biotinylated recombinant hPLAUR (uPAR) to 2 µg/mL immobilized human u-Plasminogen Activator (Urokinase) (Cat. No. 755304). ND50 range: 4 - 20 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2894531 (BioLegend Cat. No. 946203)
AB_2894531 (BioLegend Cat. No. 946204)

Antigen Details

Structure
Monomer
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes, and activated T cells

Function
Convert plasminogen to plasmin, cell adhesion, and chemotaxis
Interaction
Human PLAUR is able to bind uPA in a dose dependent manner.
Ligand/Receptor
uPA; LRP1 (CD91)
Antigen References

1. Llinas P, et al. 2005. EMBO J. 24:1655.
2. Beaufort N, et al. 2004. J. Immunol. 172:540.
3. Tarui T, et al. 2001. J. Biol. Chem. 276:3983.
4. Qin DD, et al. 2014. Lupus 0961203314558857.
5. Thuno M, et al. 2009. Dis. Markers 27:157.
6. Alfano D, et al. 2009. Front Biosci. 14:3782.
7. Spinale JM, et al. 2014. Kidney Int. 10:1038.
8. Chounta A, et al. 2014. Liver Int. 2:601.
9. Harskamp RE and Roe MT. 2014. J. Am. Heart Assoc. 5:e001431.

Gene ID
5329 View all products for this Gene ID
UniProt
View information about PLAUR on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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