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Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Recombinant Antibody

Pricing & Availability
Clone
QA18A10 (See other available formats)
Regulatory Status
RUO
Other Names
TGFbeta,TGFB, TGF-Beta-1, TGF-Beta1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A10_ULEAF_TGF-beta1_RecomAntibody_022422_updated2
Recombinant human TGF-β1 (Cat. No. 781802) (black circles) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 (clone QA18A10) (purple circles) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.05 – 0.25 µg/mL
  • QA18A10_ULEAF_TGF-beta1_RecomAntibody_022422_updated2
    Recombinant human TGF-β1 (Cat. No. 781802) (black circles) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 (clone QA18A10) (purple circles) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.05 – 0.25 µg/mL
Cat # Size Price Quantity Check Availability Save
947303 100 µg 287€
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947304 1 mg 964€
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Description

TGF-β1 is synthesized in cells as a 390-amino acid. Furin cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide (LAP), and the 25-kD C-terminal portion of the precursor constitutes the mature TGF-β1. TGF-β1 activators can release TGF-β1 from LAP. These activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and integrins avb6 and avb8. Mouse TGF-β1 converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORγt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORγt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2 in combination with IL-6, IL-21 or IL-23.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Human TGF-β1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the inhibition of HT-2 cell proliferation induced by recombinant human TGF-β1 (0.4 ng/mL, Cat. No. 781802). ND50 range: 0.05 – 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910510 (BioLegend Cat. No. 947303)
AB_2910510 (BioLegend Cat. No. 947304)

Antigen Details

Structure
Homodimer
Distribution

TGF-β1 is secreted by numerous cells

Function
Regulation of cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, and tissue remodeling and repair. Essential for wound healing, stimulates matrix molecule deposition and angiogenesis, and is an essential mediator of the pathologic scarring in fibrotic disorders
Interaction
Embryonic stem cells, endothelial cells, fibroblasts, mesenchymal cells, mesenchymal stem cells, neural stem cells, osteoclasts, Th17, Tregs
Ligand/Receptor
TGF-β1 binds to type II and type I serine/threonine kinase receptors, which initiate intracellular signals through activation of Smad proteins.
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Sun X, et al. 2013. J Virol. 87:10126-38.
  2. Benlhabib H, et al. 2015. J Biol Chem. 290:22409-22.
  3. Lin C, et al. 2016. J Exp Med. 213:251-71.
  4. Chen P, et al. 2016. Sci Rep. 6:33407.
  5. de la Mare J, et al. 2017. BMC Cancer. 17:202.
  6. Du C, et al. 2017. Int J Biochem Cell Biol. 90:17-28.
  7. Nüchel J, et al. 2018. Autophagy. 14:465-486.
Gene ID
7040 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02/24/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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