Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Recombinant Antibody

Pricing & Availability
Clone
QA18A10 (See other available formats)
Regulatory Status
RUO
Other Names
TGFbeta,TGFB, TGF-Beta-1, TGF-Beta1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A10_ULEAF_TGF-beta1_RecomAntibody_022422_updated2
Recombinant human TGF-β1 (Cat. No. 781802) (black circles) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 (clone QA18A10) (purple circles) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.05 – 0.25 µg/mL
  • QA18A10_ULEAF_TGF-beta1_RecomAntibody_022422_updated2
    Recombinant human TGF-β1 (Cat. No. 781802) (black circles) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 (clone QA18A10) (purple circles) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.05 – 0.25 µg/mL
Cat # Size Price Quantity Check Availability Save
947303 100 µg 287€
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947304 1 mg 964€
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Description

TGF-β1 is synthesized in cells as a 390-amino acid. Furin cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide (LAP), and the 25-kD C-terminal portion of the precursor constitutes the mature TGF-β1. TGF-β1 activators can release TGF-β1 from LAP. These activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and integrins avb6 and avb8. Mouse TGF-β1 converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORγt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORγt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2 in combination with IL-6, IL-21 or IL-23.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Human TGF-β1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the inhibition of HT-2 cell proliferation induced by recombinant human TGF-β1 (0.4 ng/mL, Cat. No. 781802). ND50 range: 0.05 – 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910510 (BioLegend Cat. No. 947303)
AB_2910510 (BioLegend Cat. No. 947304)

Antigen Details

Structure
Homodimer
Distribution

TGF-β1 is secreted by numerous cells

Function
Regulation of cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, and tissue remodeling and repair. Essential for wound healing, stimulates matrix molecule deposition and angiogenesis, and is an essential mediator of the pathologic scarring in fibrotic disorders
Interaction
Embryonic stem cells, endothelial cells, fibroblasts, mesenchymal cells, mesenchymal stem cells, neural stem cells, osteoclasts, Th17, Tregs
Ligand/Receptor
TGF-β1 binds to type II and type I serine/threonine kinase receptors, which initiate intracellular signals through activation of Smad proteins.
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Sun X, et al. 2013. J Virol. 87:10126-38.
  2. Benlhabib H, et al. 2015. J Biol Chem. 290:22409-22.
  3. Lin C, et al. 2016. J Exp Med. 213:251-71.
  4. Chen P, et al. 2016. Sci Rep. 6:33407.
  5. de la Mare J, et al. 2017. BMC Cancer. 17:202.
  6. Du C, et al. 2017. Int J Biochem Cell Biol. 90:17-28.
  7. Nüchel J, et al. 2018. Autophagy. 14:465-486.
Gene ID
7040 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02/24/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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  • Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Recombinant Antibody

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