Tau Antibody Sampler Kit

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Tau_Antibody_sampler_1_041618
IHC staining of anti-Tau Phospho (Ser262) antibody (clone A15091A) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with the primary antibody at 10 µg/ml for 2 hours at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The antibody specificity was confirmed by inhibiting its staining by pre-incubating the mAb with a 10-fold molar excess of pS262 tau peptide-BSA. In contrast, incubation with a 10-fold molar excess of a pT181 Tau peptide-BSA did not alter the antibody’s activity.
  • Tau_Antibody_sampler_1_041618
    IHC staining of anti-Tau Phospho (Ser262) antibody (clone A15091A) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with the primary antibody at 10 µg/ml for 2 hours at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The antibody specificity was confirmed by inhibiting its staining by pre-incubating the mAb with a 10-fold molar excess of pS262 tau peptide-BSA. In contrast, incubation with a 10-fold molar excess of a pT181 Tau peptide-BSA did not alter the antibody’s activity.
  • Tau_Antibody_sampler_2_041618
    IHC staining of anti-Tau, 1-223 antibody (clone A16103A) and rat IgG2b isotype control on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with anti-Tau or rat IgG2b at 0.2 µg/ml for 1 hour at room temperature. Biotinylated anti-rat IgG, HRP Streptavidin, and DAB (3,3'-diaminobenzidine) substrate were used as the detection system. Slides were counterstained with hematoxylin.
  • Tau_Antibody_sampler_3_041618
    IHC staining of anti-Tau, 368-441 antibody (clone A161097F) and rat IgG2a isotype control on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with 5.0 µg/ml of anti-Tau or rat IgG2a overnight at 4°C. Biotinylated anti-rat IgG, HRP Streptavidin, and DAB (3,3'-diaminobenzidine) substrate were used as the detection system. Slides were counterstained with hematoxylin.
  • Tau_Antibody_sampler_4_041618
    Western blot of anti-Tau Phospho (Thr181) (clone M7004D06), anti-Tau Phospho (Ser396) (clone PHF-13) antibodies, and isotype-matched control IgG1 and IgG2b. Lane 1: 20 µg of Parkinson's disease human brain lysate; Lane 2: 2 µg of Tau paired helical filaments. The blots were incubated overnight at 4°C with 10 µg/ml of the primary antibody, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Western blot of anti-Tau Phospho (Ser396) (clone PHF-13) serves as a positive control for Tau staining.
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899902 1 kit 244€
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development. Abnormal hyperphosphorylation, aggregation, and toxic gain of function of tau are associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Phosphorylated tau at serine 262 is commonly associated with AD legion sites. Recent studies indicate that cerebrospinal fluid tau phosphorylated at threonine 181 has diagnostic utility for several neurological disorders including AD. The Tau Antibody Sampler Kit provides flexibility for sampling and detection of phosho-specific species of tau and total tau in human tissue.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Format Clone Size Reactivity Isotype
Anti-Tau Phospho (Ser262) Purified A15091A 25 μg Human Mouse IgG2b, κ
Anti-Tau Phospho (Thr181) Purified M7004D06 25 μg Human Mouse IgG1, κ
Anti-Tau, 1-223 Purified A16103A 25 μg Human Rat IgG2b, κ
Anti-Tau, 368-441 Purified A16097F 25 μg Human Rat IgG2a, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Reactivity
Human
Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

IHC-P, WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by immunohistochemical staining on formalin-fixed paraffin-embedded tissue or Western blotting. For immunohistochemistry, the suggested uses of these reagents are as follows:

Anti-Tau Phospho (Ser262): 5.0 - 10.0 µg/ml
Anti-Tau Phospho (Thr181): 1.0 - 10.0 µg/ml

For Western blotting, the suggested uses of these reagents are as follows:

Anti-Tau, 1-223: 1.0 - 5.0 µg/ml
Anti-Tau, 368-441: 1.0 - 5.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

RRID
AB_2734652 (BioLegend Cat. No. 899902)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, Tau
Antigen References

1. Frontini M, et al. 2009. Nucleic Acids Res. 37:1073.
2. Yarilina A, et al. 2008. Nat. Immunol. 9:378.
3. Hayashi H, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:18766.
4. Hida S, et al. 2005. Blood. 106:2011.
5. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
6. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
7. Lu R. 2008. Trends Immunol 29:487.
8. Barnes BJ, et al. 2003. J. Biol. Chem. 278:16630.
9. Rullo OJ, et al. 2010. Ann. Rheum. Dis. 69:611.
10. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
11. Restivo G, et al. 2011. EMBO 30:4571.
12. Yu Y, et al. 2010. Immunity. 33:863.
13. Liang Q, et al. 2011. J. Immunol. 186:1001.
14. Ouyang X, et al. 2011. Nat. Commun. 2:314.
15. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417.
16. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033.
17. Xiao W, et al. 2001. J. Biol. Chem. 276:23275.

Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau on UniProt.org

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