Spark YG™ 570 anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
SMI 94 (See other available formats)
Other Names
Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI94_Pure_MyelinBasicProtein_Antibody_1_033121.png
IHC staining of Spark YG™ 570 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI94_Pure_MyelinBasicProtein_Antibody_1_033121.png
    IHC staining of Spark YG™ 570 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI94_Pure_MyelinBasicProtein_Antibody_2_033121.png
    IHC staining of Spark YG™ 570 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • SMI94_Pure_MyelinBasicProtein_Antibody_3_033121.png
    IHC staining of Spark YG™ 570 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
See Spark YG™ 570 spectral data
Cat # Size Price Quantity Avail. Save
836509 25 µg 104€
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836510 100 µg 220€
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence.

SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Application References
  1. Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC)
  2. Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P)
  3. Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB)
  4. McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F)
  5. Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P)
  6. Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC)
  7. Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P)
  8. Rusielewicz T, et. al. 2014. Glia 62:580. (ICC)
  9. Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC)
  10. Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P)
  11. Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
  12. Zhao X, et al. 2010. Neuron. 65:612. (ICC)

Antigen Details

Structure
Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution

Cytoskeleton, plasma membrane, cytosol, and extracellular.

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/31/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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