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- Regulatory Status
- RUO
- Other Names
- Osteoprotegerin (OPG), Osteoclastogenesis inhibitory factor (OCIF)
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- Product Citations
- publications
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Mouse TNFRSF11B (OPG) inhibits the cytotoxic effect of recombinant human TRAIL (Cat. No. 752904) on L929 cells. The ED50 for this effect is 9.5 - 38 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 552602 | 10 µg | 72€ | ||||
| 552604 | 25 µg | 118€ | ||||
TNFRSF11B is a member of the TNFR family, which lacks the transmembrane domain and acts as a secreted decoy receptor with no direct signaling capacity. It plays a key role in the physiological regulation of osteoclastic bone resorption, postnatal bone formation, and pathological calcification of arteries. TNFRSF11B binds to its natural ligand, RANKL (OPGL), which prevents the interaction of RANKL with its receptor, present on osteoclasts, therefore inhibiting osteoclast differentiation, activation, and survival. Overexpression of TNFRSF11B in transgenic mice leads to deep osteoporosis secondary to an almost total lack of osteoclasts. In addition, TNFRSF11B-deficient mice develop early onset osteoporosis and calcification of the aorta and renal arteries, sites which have endogenous TNFRSF11B expression in normal animals. EBF family member, EBF2 activates TNFRSF11B gene transcription in a synergistic manner with Wnt/β-catenin signaling transcription factor LEF1. In addition to RANKL, TNFRSF11B binds and neutralizes the pro-apoptotic actions of TNF-related apoptosis-inducing ligand (TRAIL) expressed by vascular smooth muscle cells (VSMCs) and T cells. Lastly, TNFRSF11B is involved in B cell development and function. High levels of TNFRSF11B have been detected in serum of patients with cardiovascular diseases, diabetes, and chronic renal failure.
Product DetailsProduct Details
- Source
- Mouse TNFRSF11B, amino acids (Glu22-Leu401) (Accession# NM_008764), was expressed in 293E cells. Human IgG Fc-linker sequence-6His tag is at the carboxy terminus.
- Molecular Mass
- The 620 amino acid recombinant protein has a predicted molecular mass of approximately 70.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 100 kD and 200 kD, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Glu.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 100 µg/mL.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Mouse TNFRSF11B (OPG)-Fc chimera inhibits the cytotoxic effect of recombinant human TRAIL (Cat. No. 752902) on L929 cells. The ED50 for this effect is 9.5 – 38 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Distribution
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Osteoblasts, marrow stromal cells, endothelial and vascular smooth muscle cells. Heart, kidney, liver, spleen, and bone marrow.
- Function
- Regulation of osteoclastic bone resorption, protects large blood vessels from medial calcification. Regulation of B cell development and function. Wnt/β-catenin signaling regulates the expression of TNFRSF11B.
- Interaction
- Osteoblasts, stromal cells, T cells, and VSMCs.
- Ligand/Receptor
- RANKL (OPGL), TRAIL.
- Bioactivity
- Inhibits cytotoxic effect of human TRAIL on L929 cells.
- Biology Area
- Cancer Biomarkers, Immunology
- Molecular Family
- Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
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1. Simonet WS, et al. 1997. Cell 89:309.
2. Bucay N, et al. 1998. Genes Dev. 12:1260.
3. Emery JG, et al. 1998. J. Biol. Chem. 273:14363.
4. Yun TJ, et al. 2001. J. Immunol. 166:1482.
5. Gass DA, et al. 2005. Dev. Cell 8:751.
6. Kieslinger M, et al. 2005. Dev. Cell 9:757.
7. Boyce BF and Xing L. 2007. Arthritis Res. Ther. 9: S1.
8. Van Campenhout A and Golledge J. 2009. Atherosclerosis 204:321. - Gene ID
- 18383 View all products for this Gene ID
- UniProt
- View information about TNFRSF11B on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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