Recombinant Mouse Prolactin (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Luteotropin, PRL
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Product Citations
publications
Prolactin_Mouse_RECOM_CF_BA_062716
Mouse Prolactin induces the proliferation of Nb2-11 rat lymphoma cells.
  • Prolactin_Mouse_RECOM_CF_BA_062716
    Mouse Prolactin induces the proliferation of Nb2-11 rat lymphoma cells.
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757902 10 µg 60,00€
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757904 25 µg 116,00€
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757906 100 µg 236,00€
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757908 500 µg 716,00€
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Description

Prolactin is a hormone that is secreted by the pituitary gland. Besides the pituitary origin, prolactin is found in other tissues and cells such as adipocytes, immune cells, skin, prostate, brain, and the decidua. It is best known for its role in enabling female mammals to produce milk. However, prolactin also plays essential roles in the regulation of immune functions, pancreatic development, angiogenesis, reproduction, osmoregulation, and metabolism. This hormone is produced in increasing amounts during pregnancy and suckling, and it acts on the mammary gland by initiating and maintaining lactation in the postpartum period. Mouse prolactin has a 61% amino acid sequence homology with its human counterpart. In a knockout animal study, it was shown that female mice lacking the expression of prolactin are infertile. Nevertheless, they manifest spontaneous maternal behaviors. In addition, these animals show no sign of alterations in myelopoiesis and lymphopoiesis, suggesting that prolactin may not play an indispensable role in lymphocyte and myeloid development and myeloid differentiation. Epidemiological studies have demonstrated that circulating high levels of prolactin increase the risk of breast cancer. Prolactin may promote mammary tumor initiation via the JAK2/STAT5 signaling pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse Prolactin, amino acids Met-(Leu32-Cys228) (Accession# NM_011164), was expressed in E.coli.
Molecular Mass
The 198 amino acid recombinant protein has a predicted molecular mass of approximately 22.5 kD. The protein migrates at approximately 22 kD under DTT-reducing conditions and at approximately 20 kD under non-reducing conditions respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Mouse Prolactin induces the proliferation of Nb2-11 rat lymphoma cells in a dose dependent manner. The ED50 = 0.2 - 1.0 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Kogata N, et al. 2018. Commun Biol. 1:228. PubMed

Antigen Details

Structure
Monomer.
Distribution
Anterior pituitary (lactotrophs), immune cells, brain, decidua, adipocytes, and cholangeocytes.
Function
Lactogenic activity and breast and prostate cancer development. Prolactin secretion is negatively controlled by hypothalamic dopamine. Prolactin releasing factors include the thyrotropin-releasing hormone, vasoactive intestinal polypeptide, vasopressin, serotonin, angiotensin II, and oxytocin.
Interaction
Mammary epithelial cells.
Ligand/Receptor
PRLR (prolactin receptor).
Bioactivity
Mouse prolactin induces the proliferation of Nb2-11 rat lymphoma cells.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Harigaya T, et al. 1986. Biochim. Biophys. Acta. 1:30-8.
2. Horseman ND, et al. 1997. EMBO J. 16:6926.
3. Ben-Jonathan N, et al. 1996. Endocr. Rev. 17:639.
4. Ben-Jonathan N, et al. 2008. Endocr. Rev. 1:1-41.
5. Bernichtein S, et al. 2010. J. Endocrinol. 206:1.
6. Correale J, et al. 2014. J. Neuroimmunol. 269:76.
7. Kennett JE, McKee DT. 2012. J. Neuroendocrinol. 24:403.

Gene ID
19109 View all products for this Gene ID
UniProt
View information about Prolactin on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 06/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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