Recombinant Mouse IL-6Rα-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-6RA, IL6RA, CD126, Gp80, IL-6R-1, IL6R, IL-6R-Alpha, IL-6R Subunit Alpha
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Mouse_IL-6Ralpha-Fc_Chimera_CF_RECOM_1_060721.png
When recombinant mouse IL-6Rα-Fc chimera is immobilized at 1 µg/mL, recombinant mouse IL-6 (Cat. No. 575702) binds to mouse IL-6Rα-Fc chimera in a dose-dependent manner. The ED50 for this effect is 12 – 48 ng/mL.
  • Mouse_IL-6Ralpha-Fc_Chimera_CF_RECOM_1_060721.png
    When recombinant mouse IL-6Rα-Fc chimera is immobilized at 1 µg/mL, recombinant mouse IL-6 (Cat. No. 575702) binds to mouse IL-6Rα-Fc chimera in a dose-dependent manner. The ED50 for this effect is 12 – 48 ng/mL.
  • Mouse_IL-6Ralpha-Fc_Chimera_CF_RECOM_2_060721.png
    Stability Testing for Recombinant Mouse IL-6Rα-Fc Chimera. Recombinant mouse IL-6Rα-Fc chimera was aliquoted in PBS pH 7.5. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested by functional assay. Recombinant mouse IL-6 (Cat. No. 575702) binds to immobilized recombinant mouse IL-6Rα-Fc chimera in a dose-dependent manner. The ED50 for this effect is 12 – 48 ng/mL.
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795204 25 µg 199€
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795206 100 µg 634€
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Description

IL-6 is a cytokine that plays multiple roles in inflammation, acute phase response, hematopoiesis, and regulation of the immune system. IL-6 binds to IL-6Rα with nanomolar affinity. The complex IL-6/IL-6Rα triggers dimerization of gp130, in which the receptor-ligand interaction induces the signal transduction though the JAK/STAT pathway. gp130 is also a receptor subunit for IL-11, CNTF, LIF, OSM, CT-1, CLC, and IL-27. gp130 is ubiquitously expressed in different types of cells. Nevertheless, gp130 does not bind IL-6 nor IL-6Rα, thus cells only expressing gp130 are unresponsive to IL-6. IL-6Rα and gp130 have soluble forms. Soluble IL-6Rα (sIL-6Rα) is generated by two mechanisms: by shedding from the membrane and by translation of a differentially spliced RNA. Cells expressing only gp130 can be responsive to the complex of IL-6/sIL-6Rα, and this process has been named trans-signaling. Inflammatory processes induce the shedding of IL-6R from neutrophils, and the complex IL-6/sIL-6Rα activates endothelial cells to produce MCP-1, which attracts monocytes. Soluble gp130 (sgp130) can be found in a ternary complex with IL-6 and sIL-6Rα, and it has been suggested to be the natural inhibitor of sIL-6Rα. Increased levels of sIL-6Rα have been detected in colitis, arthritis, and idiopathic pulmonary fibrosis. In vivo neutralization of sIL-6Rα attenuated pulmonary fibrosis in mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse IL-6Rα, amino acid (Leu20-Glu357) (Accession: # P22272), was expressed in CHO cells. The carboxy terminus contains SR-IEGRMD-mIgG2a-Fc (Pro99-Lys330) tag.
Molecular Mass
The 578 amino acid recombinant protein has a predicted molecular mass of approximately 64.6 kD. The DTT-reduced and non-reduced protein migrates at approximately 70 and 140 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant mouse IL-6Rα-Fc chimera is immobilized at 1 µg/mL, recombinant mouse IL-6 (Cat. No.575702) binds to mouse IL-6Rα-Fc chimera in a dose-dependent manner. The ED50 for this effect is 12 – 48 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

IL-6Rα is expressed in hepatocytes, neutrophils, macrophages, and T cells. Soluble IL-6Rα is present in body fluids (urine and blood).

Function
Soluble IL-6Rα plays a key role in IL-6 trans-signaling, which is a process that allows the response to IL-6 on cells that do not express IL-6Rα. It increases the polarization of Th17 by IL-6. It is produced by proteolysis by ADAM10 and ADAM17 in monocytes. Its shedding is induced by PMA, IL-1β, TNF-α, and apoptosis.
Interaction
Endothelial cells and a variety of cells expressing gp130
Ligand/Receptor
IL-6 and sIL-6Rα/IL-6 complex bind cells expressing gp130
Bioactivity
When recombinant mouse IL-6Rα-Fc chimera is immobilized at 1 µg/mL, recombinant mouse IL-6 (Cat. No. 575702) binds to mouse IL-6Rα-Fc chimera in a dose-dependent manner.
Cell Type
Endothelial cells, Lymphocytes, Macrophages, Monocytes, Neutrophils, T cells, Th17, Tregs
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Neuroinflammation
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Jostock T, et al. 2001. Eur J Biochem. 1:160.
  2. Saito T, et al. 1991. J Immunol. 147:168.
  3. Vermes C, et al. 2002. J Biol Chem. 277:16879.
  4. Kamimura D, et al. 2003. Rev Physiol Biochem Pharmacol. 149:1.
  5. Matthews V, et al. 2003. J Biol Chem. 278:38829.
  6. Chalaris A, et al. 2007. Blood. 110:1748.
  7. Le TT, et al. 2014. J Immunol. 7:3755.
Gene ID
16194 View all products for this Gene ID
UniProt
View information about IL-6Ralpha on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 06/07/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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