Recombinant Mouse IL-33 (ELISA Std.)

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Other Names
Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
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580509 4 pack 64€
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Description

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
Molecular Mass
The 158 amino acid recombinant protein has a predicted molecular mass of approximately 17,554 Da. The DTT-reduced and non-reduced protein migrate at approximately 20 kDa by SDS-PAGE. The N-terminal amino acid is Serine.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Endotoxin level is < 0.1 EU/µg (< 0.01ng/µg) protein as determined by the LAL method.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant mouse IL-33 at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000 pg/ml to 15.6 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-33 protein is useful as a standard in a mouse IL-33 sandwich ELISA, using unlabeled poly5165 antibody (Cat. No. 516501) for capture and biotinylated poly5165 antibody (Cat. No. 516503) for detection.

Application References
  1. Gao X, et al. 2015. J Immunol. 194:438. PubMed
Product Citations
  1. Gao X, et al. 2015. J Immunol. 194:438. PubMed

Antigen Details

Distribution

High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.

Function
IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes.
Ligand/Receptor
IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
Bioactivity
Activity was tested by induction of IL-5 in activated splenocytes by IL-33. The ED50 is 2 to 5 ng/ml, corresponding to a specific activity of 5 to 2 x 105 units/mg. Under this assay the activity was comparable to other vendor's cytokine.
Biology Area
Stem Cells, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schmitz J, et al. 2005. Immunity 23:479.
2. Barksby HE, et al. 2007. C. Exp. Immunol. 149:217.
3. Arend WP, et al. 2008. Immol. Rev. 223:20.
4. Suzukawa M, et al. 2008. J. Immunol. 181:5981.
5. Moussio C, et al. 2008. PlosOne 10:e3331.

Gene ID
77125 View all products for this Gene ID
UniProt
View information about IL-33 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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