Recombinant Human IL-17A (ELISA Std.)

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Other Names
Interleukin 17A, IL17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
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570509 4 pack 64€
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Description

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells)mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA).Human IL-17A shows 63%, 63% and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.Both recombinant and natural IL-17A have been shown to exist as a disulfide-linked homodimeric glycoprotein containing two ~16 kD monomers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli and renatured from inclusion bodies.
Molecular Mass
The 137 amino acid N-terminal methionylated protein has a predicted molecular mass of 15,666 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16kDa by SDS-PAGE. The non-reduced protein migrates
Purity
Purity is >98% as determined by SDS PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant human IL-17A at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 15 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-17A protein is useful as a standard in a human IL-17A sandwich ELISA in conjunciton with the monoclonal antibody pair: unlabeled purified clone BL127 antibody (capture) and biotinylated clone BL23 antibody (detection).

Antigen Details

Structure
Cytokine; dimer
Cell Sources
activated T cell subpopulation
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CDw217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hirota K, et al. 2007. J Exp Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332.

Gene ID
NA
UniProt
View information about IL-17A on UniProt.org

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