- Regulatory Status
- Other Names
- Interleukin 16, Lymphocyte chemoattractant factor (LCF)
- Ave. Rating
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- Product Citations
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IL-16 is a proinflammatory cytokine synthesized as a 631-amino acid precursor protein (pro-IL-16). The pro-IL-16 is expressed at high level in T cells. After cell activation via the T cell rceptor, the C-terminal cytokine domain (121 amino acids) can be released from the N-terminal prodomain by caspase-3 and become biological active. Self-aggregation into homotetramer is required for its biological activities. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. IL-16 also induces the expression of CD25 and activates CD4+ T cells synergically with IL-2 or IL-15. IL-16 can also induce IL-1, IL-6 and TNF-α production in monocytes. It has been shown tha IL-16 can inhibit HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. The pro-IL-16 has been identified as a T lymphocyte cell cycle growth suppressor. In primary T cells, nuclear presence of pro-IL-16 results in a resting state. Pro-IL-16 can function as a scadffold protein which can interact with GA-binding protein β, HDAC3, and heat shock protein 70.Product Details
- Human IL-16, amino acids Ser10-Ser130 (Accession# AAC12732), was expressed in E. coli.
- Molecular Mass
- The 121 amino acid recombinant protein has a predicted molecular mass of approximately 12.4 kD. The predicted N-terminal amino acid is Ser.
- >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Lyophilized, carrier-free.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Determined by its ability to chemoattract human peripheral blood lymphocytes using a concentration range of 50.0 - 1000.0 ng/ml.
- IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, dendritic cells and fibroblasts. The release of IL-16 can be induced by Ag, mitogen, histamine, and serotonin.
- IL-16 is not only a chemoattractant but also a regulator of T cell activation and growth.
- CD4+ T cells, monocyte, eosinophils.
- CD4 and CD9.
- Biology Area
- Immunology, Innate Immunity, Stem Cells, Cell Biology
- Molecular Family
- Antigen References
1. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
2. Zhang Y, et al. 1998. J. Biol. Chem. 273: 1144.
3. Zhou P, et al. 1999. J. Immunol. 163: 906.
4. Wilson KC, et al. 2004. Growth Factors. 22:97.
5. Chupp GL, et al. 1998. J. Immunol. 161: 3114.
6. Center DM, et al. 20024. J. Immunol. 172:1654.
- Gene ID
- 3603 View all products for this Gene ID
- View information about IL-16 on UniProt.org
Related Pages & Pathways
- Why choose BioLegend recombinant proteins?
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins.
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)