Recombinant Human IL-15 (ELISA Std.)

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Other Names
Interleukin-15 (IL-15), MGC9721
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570309 4 pack 64€
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Description

Interleukin-15 (IL-15) was discovered in supernatant from simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). IL-15 is a regulatory cytokine that is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). It plays an important role in immune response and shares many functions with IL-2, for example, it stimulates the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and it induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation (9).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
E. coli expressed protein
Molecular Mass
The 114 amino acid recombinant protein has a predicted molecular mass of 12.8 kD. The DTT-reduced protein migrates at approximately 9 kD and the non-reduced protein migrates at approximately 7 kD by SDS-PAGE. The N-terminal amino acid is Asn.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 7.8 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-15 protein is useful as a standard for a human IL-15 sandwich ELISA, using unlabelled BH1509 antibody (Cat. No. 515002) for capture and biotinylated BH1543 antibody (Cat. No. 515104) for detection.

Antigen Details

Structure
13 kD single non-glycosylated polypeptide.
Distribution

Adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells.

Function
IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils, and induces the production of proinflammatory cytokines from macrophages.
Interaction
T lymphocytes, NK cells
Ligand/Receptor
IL-15Rα, IL-15Rβ and IL-15γc.
Cell Type
B cells
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Grabstein K, et al. 1994. Science 264:965.
2. Ma A, et al. 2006. Annu. Rev. Immunol. 24:657.
3. Meresse B, et al. 2004. Immunity 21:357.
4. Armitage RJ, et al. 1995. J. Immunol. 154:483.
5. Pulendran B, et al. 2004. Eur. J. Immunol. 34:66.
6. Bouchard A, et al. 2004. J. Leukoc. Biol. 76:162.
7. Ratthe C, et al. 2004. J. Leukoc. Biol. 75:893.
8. Feng T, et al. 2008. Cell Immunol. 5:189.
9. Rubinstein MP, et al. 2006. P. Natl. Acad. Sci. USA 103:9166.

Gene ID
3600 View all products for this Gene ID
UniProt
View information about IL-15 on UniProt.org

Related FAQs

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