Recombinant Human GM-CSF (ELISA Std.)

Pricing & Availability
Other Names
Colony stimulating factor 2, CSF2, Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA).
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572409 4 pack 64€
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Description

GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always coexpressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain nonhemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human GM-CSF, amino acids Ala18- Glu144 (Accession# AAA52578) was expressed in E. coli, approximately 14.6 kD.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant human GM-CSF at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This GM-CSF protein is useful as a standard for a human GM-CSF sandwich ELISA, using unlabelled anti-GM-CSF antibody, clone BVD2-23B6 (catalog #502202) for capture and biotinylated BVD2-21C11 (catalog #502304) antibody for detection.

Antigen Details

Structure
Cytokine
Distribution

T cells, macrophages, fibroblasts, endothelial cells, mast cells.

Function
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Interaction
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells.
Ligand/Receptor
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common
Bioactivity
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and the related cytokines interleukin (IL)—3 and IL-5 regulate the production and functional activation of hemopoietic cells, with GM-CSF acting on monocyte/macrophages and all granulocytes. GM-C
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Hercus TR, et al. 2009 Blood 114:1289-1298.
2. Hayashida K, et al. 1990 Proc Natl Acad Sci USA. 87:9655-9659.
3. Walker F, et al. 1985 EMBO J 4:933-939.
4. Kitamura T, et al. 1989 J. Cell Physiol. 140:323.

Gene ID
NA
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

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