Recombinant Human BDNF (ELISA Std.)

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Other Names
Brain-derived neurotrophic factor, ANON2, BLUN2
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RECOM_HU_BDNF_ELISA_STD_121118.PNG
  • RECOM_HU_BDNF_ELISA_STD_121118.PNG
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Description

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammals examined. All neurotrophins are noncovalently linked homodimers and initially produced as proneurotrophins. These proneurotrophins have distinct biological activity different from their mature counterparts. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during the development. BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human BDNF, amino acids His129-Arg247 (Accession# P23560) was expressed in CHO cells.
Molecular Mass
The 119 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kD. The DTT-reduced and non-reduced proteins migrate at approximately 14.5 kD by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Storage & Handling
Immediately upon receipt, store unopened vials between 2°C and 8°C and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 156.3 to 10,000 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This human BDNF protein is useful as a standard for a human BDNF sandwich ELISA, using unlabeled A15062G antibody (Cat. No. 536702) as capture and biotinylated A15062F antibody (Cat. No. 536804) as detection.

Antigen Details

Structure
Noncovalently linked homodimer
Distribution

Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung, and testis.

Function
Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
Interaction
Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons.
Ligand/Receptor
TrkB
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References
  1. Baloh RH, et al. 1998. Neuron. 21:1291
  2. Masure S, et al. 1999. Eur. J. Biochem. 266:892-902.
  3. Gardell LR, et al. 2003. Nat. Med. 9(11):1383-9.
  4. Honma Y, et al. 2002. Neuron. 35:267-82.
  5. Banerjee A, et al. 2012. PLoS One 7(11):e50098.
  6. Wong LE, et al. 2015. Proc. Natl. Acad. Sci. USA 112:6170-5.
  7. Zhang M, et al. 2016. Oncotarget. 7:3267-82.
Gene ID
627 View all products for this Gene ID
UniProt
View information about BDNF on UniProt.org

Related FAQs

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