Purified β-Amyloid, 1-15 Antibody

Pricing & Availability
Clone
3A1 (See other available formats)
Other Names
AAA, ABETA, ABPP, AD1, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
3A1_Pure_B-Amyloid_1-15_Antibody_1_032218_updated
IHC staining of purified anti-β-Amyloid, 1-15 antibody (clone 3A1) on frozen normal (left panel) and Alzheimer's disease (right panel) human brain tissues. The tissues were incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
  • 3A1_Pure_B-Amyloid_1-15_Antibody_1_032218_updated
    IHC staining of purified anti-β-Amyloid, 1-15 antibody (clone 3A1) on frozen normal (left panel) and Alzheimer's disease (right panel) human brain tissues. The tissues were incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
  • 3A1_Pure_B-Amyloid_1-15_Antibody_2_032218_updated
    Direct ELISA of purified anti-β-Amyloid, 1-15 antibody (clone 3A1) binding to plate-immobilized Aβ1-40 (left panel) and lack of reactivity with plate-immobilized recombinant APP (right panel). ELISA was performed by coating wells with 100 ng of peptide or protein. The wells were then incubated with clones 3A1, 6E10 or mouse IgG at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Clone 6E10 was included as a control for cross-reactivity with Aβ1-40 and APP. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # Size Price Quantity Avail. Save
847501 25 µg 88€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
847502 100 µg 220€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor protein (APP) through sequential proteolytic cleavage of APP by β- and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against dityrosine cross-linked human Aβ1-40.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
Direct ELISA - Validated
IHC-P - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For ELISA applications, a concentration range of 0.01 - 1.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

Application References
  1. Frost JL, et al. 2015. Neurobiol Aging. 36(12):3187 (IHC-P)
  2. Lardenoije R, et al. 2018. Mol. Cell. Neurosci. 86:1-15. (IHC-P) PubMed
RRID
AB_2728567 (BioLegend Cat. No. 847501)
AB_2728568 (BioLegend Cat. No. 847502)

Antigen Details

Structure
Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.

Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Interaction
Tau, Prion
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608.
  2. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595.
  3. Walsh DM, et a. 2007. J Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account