Purified anti-VEGF Antibody

Pricing & Availability
Clone
Poly6275 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor, vasculotropin, vascular permeability factor
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
VEGF_090105
Human recombinant VEGF 165 produced in yeast was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-VEGF polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects reduced VEGF consistent with the molecular weight shown (approximately 22-24 kD) as well as unreduced VEGF (data not shown).
  • VEGF_090105
    Human recombinant VEGF 165 produced in yeast was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-VEGF polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects reduced VEGF consistent with the molecular weight shown (approximately 22-24 kD) as well as unreduced VEGF (data not shown).
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627501 50 µl (5 Western blots) 80,00€
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Description

VEGF, also known as vascular endothelial growth factor, vasculotropin, and vascular permeability factor, is a widely expressed mitogen for vascular endothelial cells that has been shown to promote angiogenesis and increase permeability of capillary blood vessels. VEGF is a member of the platelet-derived growth factor (PDGF) family and is produced as a homodimeric protein with approximate molecular weights of 34-46 kD. The variance in molecular weight is a result of alternative splicing events that encode monomeric proteins having 121, 165, 189 or 206 amino acids. VEGF121 and VEGF165 are secreted proteins whereas VEGF189 and VEGF206 are strongly cell-associated. VEGF binds to VEGF-R1 (also known as Flt-1) and VEGF-R2 (also known as KDR). Inhibition of VEGF biding to VEGF-Rs has been the focus of a number of tumor therapeutic strategies. The Poly6275 antibody recognizes the N-terminal region of human VEGF has been shown to be useful for Western blotting of both reduced and non-reduced VEGF.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide mapping to a domain near the N-terminus
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.1% sodium azide and 0.2% gelatin.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Product Citations
  1. Jin G, et al. 2020. Oxid Med Cell Longev. 7690845:2020. PubMed
RRID
AB_2272658 (BioLegend Cat. No. 627501)

Antigen Details

Structure
Contains 8 cysteine residues involved in intra- and inter-chain disulfide bonds, platelet-derived growth factor (PDGF) family, produced as a homodimeric protein with an approximate molecular weight of 34-46 kD. Human VEGF is alternatively spliced to encod
Distribution

Widely expressed

Function
Mitogen for vascular endothelial cells, promotes angiogenesis and increases permeability of capillary blood vessels
Interaction
Heparin, many other proteins
Ligand/Receptor
VEGF-R1 (Flt-1) and VEGF-R2 (KDR)
Modification
Glycosylation
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Connolly DT, et al. 1989. J. Clin. Invest. 84:1470.
2. Drake CJ, et al. 1995. Proc. Natl. Acad. Sci. USA 92:7657.
3. Houck KA, et al. 1991. Mol. Endocrinol. 5:1806.

Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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