Purified anti-V5-tag Antibody

Pricing & Availability
Clone
7/4 (See other available formats)
Regulatory Status
RUO
Other Names
GKPIPNPLLGLDST epitope tag
Isotype
Mouse IgG2b, κ
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Product Citations
publications
7slash4_PURE_V5_Antibody_1_WB_093015
Different amounts of V5 tagged proteins (Genescript, M0101) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal V5-tag (clone 7/4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 7slash4_PURE_V5_Antibody_1_WB_093015
    Different amounts of V5 tagged proteins (Genescript, M0101) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal V5-tag (clone 7/4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 7slash4_PURE_V5_Antibody_2_IP_093015
    200ng V5 tagged protein was resuspended in PBS. Lane 1 was immunoprecipitated with 2 µg mouse IgG. Lane 2 was immunoprecipitated with 2 µg anti-V5-tag (clone 7/4) antibody. Lane 3 was immunoprecipitated with 2 µg anti-V5-tag (clone poly290238) antibody. Lane 4 was loaded with 50ng V5 tagged protein directly as a positive control. Western blot analysis was performed using mouse anti-V5 tag antibody (clone 7/4) and followed by HRP-anti-mouse IgG.
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680601 25 µg 68,00€
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680602 100 µg 180,00€
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Description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
GKPIPNPLLGLDST tag epitope
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Bussey KA, et al. 2018. PLoS Pathog. 11:43. PubMed
  2. Stempel M, et al. 2019. EMBO J. 38:. PubMed
  3. Lou Y, et al. 2020. Cell Mol Immunol. 1.439583333. PubMed
  4. Pham TH, et al. 2020. Cancer Discov. . PubMed
RRID
AB_2566386 (BioLegend Cat. No. 680601)
AB_2566387 (BioLegend Cat. No. 680602)

Antigen Details

Structure
14 amino acid peptide (GKPIPNPLLGLDST)
Function
The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
Biology Area
Cell Biology
Antigen References

1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891.

Gene ID
NA
UniProt
View information about V5-tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/30/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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