Purified anti-Tau, 1-223 Antibody

Pricing & Availability
Clone
A16103A (See other available formats)
Other Names
Microtubule associated protein tau
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
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A16103A_Purified_Tau1-223_Antibody_1_110917
Western blots of anti-Tau, 1-223 antibody (clone A16103A) and rat IgG2b isotype control. Lane 1: 20 µg of normal human brain lysate; Lane 2: 20 µg of mouse brain lysate; Lane 3: 50 ng of recombinant human Tau ladder. The blots were incubated overnight at 4°C with 1 µg/ml of anti-Tau or rat IgG2b, followed by incubation with horseradish peroxidase labeled goat anti-rat IgG secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • A16103A_Purified_Tau1-223_Antibody_1_110917
    Western blots of anti-Tau, 1-223 antibody (clone A16103A) and rat IgG2b isotype control. Lane 1: 20 µg of normal human brain lysate; Lane 2: 20 µg of mouse brain lysate; Lane 3: 50 ng of recombinant human Tau ladder. The blots were incubated overnight at 4°C with 1 µg/ml of anti-Tau or rat IgG2b, followed by incubation with horseradish peroxidase labeled goat anti-rat IgG secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • A16103A_Purified_Tau1-223_Antibody_2_110917
    IHC staining of anti-Tau, 1-223 antibody (clone A16103A) and rat IgG2b isotype control on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate, the tissues were incubated with anti-Tau or rat IgG2b at 0.2 µg/ml for 1 hour at room temperature. Biotinylated anti-rat IgG, HRP Streptavidin, and DAB (3,3'-diaminobenzidine) substrate were used as the detection system. Slides were counterstained with hematoxylin.
  • A16103A_Purified_Tau1-223_Antibody_3_110917
    Direct ELISA of purified anti-Tau, 1-223 antibody (clone A16103A) and rat IgG2b isotype control binding to plate-immobilized recombinant human 2N3R and 2N4R Tau proteins. ELISA was performed by coating wells with 100 ng of each recombinant Tau protein. The wells were then incubated with anti-Tau or rat IgG2b at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
Cat # Size Price Quantity Avail. Save
851301 25 µg 76€
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851302 100 µg 188€
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant N-terminal fragment of human 0N Tau protein encompassing amino acid residues 1-223.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, Direct ELISA - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.2 - 1.0 µg/ml is suggested. For Direct ELISA, a concentration range of 0.01 - 1.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with 3R and 4R Tau isoforms, and recognizes endogenous human Tau in brain lysates by WB. This clone does not recognize murine Tau.

RRID
AB_2721758 (BioLegend Cat. No. 851301)
AB_2721759 (BioLegend Cat. No. 851302)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed.
  3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, 1-223 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 11/17/2017

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