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Purified anti-Synaptotagmin-3 Antibody

Pricing & Availability
Clone
N278/19 (See other available formats)
Regulatory Status
RUO
Other Names
SYT3
Isotype
Mouse IgG1, κ
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Product Citations
publications
N27819_Pure_Synaptotagmin-3_Antibody_032118
Western blot of purified anti-Synaptotagmin-3 antibody (clone N278/19). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of N278/19 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N27819_Pure_Synaptotagmin-3_Antibody_032118
    Western blot of purified anti-Synaptotagmin-3 antibody (clone N278/19). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blots were incubated with 1 µg/mL of N278/19 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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852601 25 µg 67€
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852602 100 µg 132€
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Description

Synaptotagmin-3 (SYT3)  is a member of the synaptotagmin family, which consists of 13  membrane trafficking proteins that contain a N-terminal transmembrane region, and two cytoplasmic C2 domains. Synaptotagmin-3 is mainly localized at the presynaptic terminals in the central nervous system, where it functions as a Ca2+ sensor and plays an important role in exocytosis of secretory vesicles and neurotransmitter release. It has been shown that Ca2+ influx triggers the interaction between the C2-domains of SYT3 and the phospholipid membranes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 442-533 (cytoplasmic C2B domain) of mouse Synaptotagmin-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Not recommended to be used in WB with human brain lysate.

RRID
AB_2728595 (BioLegend Cat. No. 852601)
AB_2728596 (BioLegend Cat. No. 852602)

Antigen Details

Structure
Synaptotagmine-3 is a 590 amino acid protein with a molecular mass of ~ 63 kD.
Distribution

Tissue Distribution: Central Nervous System
Cellular Distribution: Endosome, plasma membrane, synaptic vesicles, and cytosol.

Function
SYT3 functions as a Ca2+ sensor at the presynaptic terminals and plays a role in Ca2+-dependent exocytosis of secretory vesicles.
Interaction
SYT3 forms homodimers and heterodimers with SYT6, SYT9 and SYT10. It binds to SNARE-complex component syntaxin-1.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Saheki Y, et al. 2017. Adv Exp Med Biol. 997:83
  2. Wang YL, et al. 2017. Cell Mol Life Sci. 74(16):2917
Gene ID
84258 View all products for this Gene ID
UniProt
View information about Synaptotagmin-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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  • Purified anti-Synaptotagmin-3

  • HRP anti-Synaptotagmin-3
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