Purified anti-RORγt Antibody

Pricing & Availability
Clone
W19344C (See other available formats)
Regulatory Status
RUO
Other Names
Rorc; Rorg; RORgamma; Rorγ; RORgammat; RORγt; TOR; Thor; Nr1f3; ROR; RAR-Related Orphan Nuclear Receptor Variant 2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19344C_PURE_RORgammat_Antibody_031122
Whole tissue lysates (15 µg total protein) from mouse thymus (positive control) and mouse spleen (negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-RORγt (clone W19344C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker
  • W19344C_PURE_RORgammat_Antibody_031122
    Whole tissue lysates (15 µg total protein) from mouse thymus (positive control) and mouse spleen (negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-RORγt (clone W19344C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker
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603151 25 µg 88€
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603152 100 µg 220€
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Description

Retinoid-related orphan receptor, or RAR-related orphan receptor-γ (RORγ) antigen, is a ligand-dependent transcription factor that regulates multiples cellular functions and processes. RORγt is an isoform that originates from alternative splicing of RORγ. While RORγ is expressed in multiple tissues and cell types, RORγt is exclusively expressed in CD4+CD8+ double positive immature T cells in the thymus. In addition, RORγt is expressed by Th17, Tc17, γd T cells, ILC3 cells, NKp46+CD3- NK cells and Lymphoid tissue inducer (LTi) cells but is absent from naïve T cells in the periphery. RORγt plays a role in thymopoiesis, circadian rhythms, tumor progression, anti-microbial immunity, and is considered as the master transcriptional regulator for Th17 differentiation. Th17 cells are recruited in response to fungal and some extracellular bacterial infections. They are also involved in the pathology of a variety of autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, and Crohn’s disease. Due to the critical role RORγt plays in Th17 differentiation, it is considered as a target of interest to treat autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse RORγt fragment
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910480 (BioLegend Cat. No. 603151)
AB_2910480 (BioLegend Cat. No. 603152)

Antigen Details

Structure
RORγt is a 518 amino acid protein with a predicted molecular weight of ~58 KD
Distribution

Expressed in the nucleus

Function
Master regulator of Th17 cells
Interaction
Interacts with coactivators NCOA1 and PPARGC1A and corepressor NCOR1
Cell Type
T cells, Th17
Biology Area
Cancer Biomarkers, Immunology, Innate Immunity, Transcription Factors
Antigen References
  1. Ivanov I, et al. 2006. Cell.126:1121-33.
  2. Medvedev A, et al. 1996. Gene. 181:199-206.
  3. Volpe E, et al. 2008. Nat Immunol. 9:650-7.
  4. Villey I, et al.1999. Eur J Immunol.29:4072-80.
  5. Castro G, et al. 2017. PLoS One. 12:e0181868.
Gene ID
6097 View all products for this Gene ID
UniProt
View information about RORgammat on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/25/2022

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  • Purified anti-RORγt

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