Purified anti-rat CD252 (OX-40 Ligand) Antibody

Pricing & Availability
Clone
ATM-2 (See other available formats)
Regulatory Status
RUO
Other Names
OX-40 Ligand, CD134L, TNFSF4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ATM-2
PMA-stimulated (2 days) Lou rat splenocytes stained with ATM-2 biotin, then detected with Sav-PE
  • ATM-2
    PMA-stimulated (2 days) Lou rat splenocytes stained with ATM-2 biotin, then detected with Sav-PE
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200501 50 µg 64,00€
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Description

OX-40 Ligand (OX-40L) is a 22 kD member of the TNF superfamily (TNFSF4), also known as CD252 or CD134L. It is expressed on dendritic cells, peritoneal macrophages, and activated B cells. The interaction of OX-40L with OX-40 (CD134) is important for B cell antibody production and T cell activation and proliferation. The ATM-2 antibody is able to block the costimulatory function of OX-40L.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ACI-S1 rat T-cell lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking the binding of OX-40 to OX-40L and the inhibition of T cell costimulatory activity of OX40L1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 200506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 200504) or biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200508).

Application References
  1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
RRID
AB_2571881 (BioLegend Cat. No. 200501)

Antigen Details

Structure
TNF superfamily, 22 kD
Distribution
Dendritic cells, activated B cells
Function
T cell dependent humoral responses
Ligand/Receptor
OX40 (CD134)
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
2. Akiba, H., et al. 1998. Biochem. Biophys. Res. Commun. 251:131.
3. Akiba, H., et al. 2000. J. Exp. Med. 191:375.

Gene ID
22164 View all products for this Gene ID
UniProt
View information about CD252 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/17/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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