Purified anti-p62 (SQSTM1) Antibody

Pricing & Availability
Clone
O95A2 (See other available formats)
Regulatory Status
RUO
Other Names
Sequestosome 1, p62, Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa, EBI3-Associated Protein Of 60 KDa, Ubiquitin-Binding Protein p62, Oxidative Stress Induced Like, Autophagy Receptor P62
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O95A2_PURE_p62_Antibody_1_050620.png
Non-targeting siRNA-treated HeLa cells were grown in normal media (panel A) or media treated with 50 µM chloroquine for 18 hours (panel B). As a negative control, HeLa cells were depleted of p62 (SQSTM1) protein using siRNA directed against SQSTM1 and grown in normal media (panel C) or media treated with 50 µM chloroquine for 18 hours (panel D). Cells were then fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 2.0 µg/mL (1:250 dilution) of purified anti-p62 (SQSTM1) antibody (clone O95A2) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 1:200 dilution. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • O95A2_PURE_p62_Antibody_1_050620.png
    Non-targeting siRNA-treated HeLa cells were grown in normal media (panel A) or media treated with 50 µM chloroquine for 18 hours (panel B). As a negative control, HeLa cells were depleted of p62 (SQSTM1) protein using siRNA directed against SQSTM1 and grown in normal media (panel C) or media treated with 50 µM chloroquine for 18 hours (panel D). Cells were then fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 2.0 µg/mL (1:250 dilution) of purified anti-p62 (SQSTM1) antibody (clone O95A2) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 1:200 dilution. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
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937201 25 µg 68,00€
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937202 100 µg 172,00€
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Description

In eukaryotes, autophagy plays a critical role in the maintenance of cellular proteostasis. Sequestosome-1 (SQSTM1), or p62 functions as a receptor during selective macroautophagy, where it is responsible for recognition and loading of misfolded proteins and dysfunctional organelles into autophagosomes for cytosolic clearance and recycling. p62 contains a ubiquitin-association (UBA) domain which directly binds ubiquitinated protein aggregate substrates. Under normal conditions, p62 exists as a dimer with the UBA domain contributing to the dimer interface. Upon phosphorylation and acetylation at residues embedded within the UBA, dimerization is disrupted, allowing the UBA to bind ubiquitinated substrates for clearance. In addition to the UBA domain, p62 contains an LC3-interacting domain that facilitates interaction with the autophagosome membrane adaptor proteins LC3 and ATG8. In addition to its role in autophagy, p62 can also function as a scaffold signaling hub and positively regulate the mTORC1, NF-κB, and Keap1-Nrf2 signaling pathways. Dysregulation of p62 and autophagy is associated with a broad spectrum of diseases, including cancer, neurodegenerative, and metabolic diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human SQSTM1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When this clone was tested for western blot, a non-specific band close to the predicted molecular weight of p62 was observed. This protein was also detected in O95A2 immunoprecipitates. We therefore do not recommend this clone for western blot or immunoprecipitation applications.

This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with Triton X-100 or methanol. Both methods were compatible with p62 staining.

RRID
AB_2861124 (BioLegend Cat. No. 937201)
AB_2861125 (BioLegend Cat. No. 937202)

Antigen Details

Structure
p62 is a 440 amino acid protein with a predicted molecular weight of 47 kD.
Distribution

Vesicles and Cytosol/Ubiquitously expressed

Function
Autophagy
Biology Area
Cell Biology, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Adaptor Proteins
Antigen References
  1. Komatsu M, et al. 2007. Cell. 6:1149.
  2. Bjørkøy G, et al. 2006. Autophagy. 2:138.
  3. Bjørkøy G, et al. 2005. J Cell Biol. 171:603.
Gene ID
8878 View all products for this Gene ID
UniProt
View information about p62 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/06/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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