- O95A2 (See other available formats)
- Regulatory Status
- Other Names
- Sequestosome 1, p62, Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa, EBI3-Associated Protein Of 60 KDa, Ubiquitin-Binding Protein p62, Oxidative Stress Induced Like, Autophagy Receptor P62
- Mouse IgG1, κ
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- Product Citations
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In eukaryotes, autophagy plays a critical role in the maintenance of cellular proteostasis. Sequestosome-1 (SQSTM1), or p62 functions as a receptor during selective macroautophagy, where it is responsible for recognition and loading of misfolded proteins and dysfunctional organelles into autophagosomes for cytosolic clearance and recycling. p62 contains a ubiquitin-association (UBA) domain which directly binds ubiquitinated protein aggregate substrates. Under normal conditions, p62 exists as a dimer with the UBA domain contributing to the dimer interface. Upon phosphorylation and acetylation at residues embedded within the UBA, dimerization is disrupted, allowing the UBA to bind ubiquitinated substrates for clearance. In addition to the UBA domain, p62 contains an LC3-interacting domain that facilitates interaction with the autophagosome membrane adaptor proteins LC3 and ATG8. In addition to its role in autophagy, p62 can also function as a scaffold signaling hub and positively regulate the mTORC1, NF-κB, and Keap1-Nrf2 signaling pathways. Dysregulation of p62 and autophagy is associated with a broad spectrum of diseases, including cancer, neurodegenerative, and metabolic diseases.Product Details
- Antibody Type
- Host Species
- Full-length recombinant human SQSTM1 protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
When this clone was tested for western blot, a non-specific band close to the predicted molecular weight of p62 was observed. This protein was also detected in O95A2 immunoprecipitates. We therefore do not recommend this clone for western blot or immunoprecipitation applications.
This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with Triton X-100 or methanol. Both methods were compatible with p62 staining.
AB_2861124 (BioLegend Cat. No. 937201)
AB_2861125 (BioLegend Cat. No. 937202)
- p62 is a 440 amino acid protein with a predicted molecular weight of 47 kD.
Vesicles and Cytosol/Ubiquitously expressed
- Biology Area
- Cell Biology, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Adaptor Proteins
- Antigen References
- Komatsu M, et al. 2007. Cell. 6:1149.
- Bjørkøy G, et al. 2006. Autophagy. 2:138.
- Bjørkøy G, et al. 2005. J Cell Biol. 171:603.
- Gene ID
- 8878 View all products for this Gene ID
- View information about p62 on UniProt.org
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