Purified anti-p38 MAPK Antibody

Pricing & Availability
Clone
Poly6224 (See other available formats)
Regulatory Status
RUO
Other Names
Stress activated kinase p38, p38 MAP kinase, stress activated protein kinase 2A
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6224_PURE_p38_MAPK_Antibody_1_072120.png
Whole cell extracts (15 µg protein) from NTERA-2, HeLa, Jurkat and NIH/3T3 cells were resolved by electrophoresis (4-12% Bis-Tris), transferred to PVDF, and probed overnight with a 1:4000 dilution of purified anti-p38 MAPK antibody (clone Poly6224). Proteins were visualized by chemiluminescent detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control. Lane M: Molecular weight ladder.
  • Poly6224_PURE_p38_MAPK_Antibody_1_072120.png
    Whole cell extracts (15 µg protein) from NTERA-2, HeLa, Jurkat and NIH/3T3 cells were resolved by electrophoresis (4-12% Bis-Tris), transferred to PVDF, and probed overnight with a 1:4000 dilution of purified anti-p38 MAPK antibody (clone Poly6224). Proteins were visualized by chemiluminescent detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control. Lane M: Molecular weight ladder.
  • Poly6224_PURE_p38_MAPK_Antibody_2_072120.png
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with 1X Citrate Buffer (diluted from 10X Citrate Buffer, Cat. No. 420902) at 95°C for 40 minutes. Tissue was then stained with a 1:200 dilution of purified anti-p38 MAPK antibody (clone Poly6224) at 4°C overnight, followed by 2.5 µg/mL (1:250 dilution) of Alexa Fluor® 594 donkey anti-rabbit IgG (Cat. No. 406418) antibody for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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622403 50 µL 80,00€
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Description

p38 mitogen-activated protein kinase (MAPK) is a member of the stress activated kinase family with a molecular weight of 38 kD. MAPK can be activated by a number of stimuli including heat, changes in osmolarity, irradiation, and cold. The MAPKs require dual phosphorylation (threonine and tyrosine) for enzymatic activation. The p38 MAP kinase is required for G2 cell cycle delay after UV irradiation, also participates in cytokine production (IL-6 and erythropoietin). The Poly6224 antibody recognizes human, mouse, and rat p38 MAPK and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
peptide-KLH, C terminal
Formulation
PBS containing 0.1% sodium azide and 0.2% gelatin
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:200 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Application References
  1. da Silva, et al. 2011. Biochim Biophys Acta. 181:1863. PubMed
  2. Chen W, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:279. PubMed
  3. Maeda S, et al. 2014. J Biol Chem. 289:23786. PubMed
RRID
AB_2876740 (BioLegend Cat. No. 622403)

Antigen Details

Structure
Belongs to serine/threonine kinase family, MAP subfamily, 38 kD
Distribution

Ubiquitously expressed, nuclear and cytoplasmic

Function
p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock-out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2, MEF2C and MAX.
Interaction
Interacts with MAX, Cdc25B, Cdc25C. Binds to kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm.
Modification
Phosphorylation
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Bulavin DV, et al. 2001. Nature 411:102.
2. Han J, et al. 1994. Science 265:808.
3. Lee JC, et al. 1994. Nature 372:739.

Regulation
Activated by environmental stress, many pro-inflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK.
Gene ID
80824 View all products for this Gene ID
UniProt
View information about p38 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/21/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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