Purified anti-mouse H-2Ld/H-2Db Antibody

Pricing & Availability
28-14-8 (See other available formats)
Regulatory Status
Other Names
MHC class I
Mouse (C3H/HeJ) IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes stained with 28-14-8 PE
  • 28-14-8
    C57BL/6 mouse splenocytes stained with 28-14-8 PE
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114502 500 µg 198€
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The 28-14-8 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2Ld haplotype. Clone 28-14-8 recognizes the α3 domain of H-2Db.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
C3H.SW mouse splenocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114510).

Does not react with other haplotypes (e.g., k, f, p, r, s).

Application References
  1. Ozato K, et al. 1980. J. Immunol. 125:2473.
  2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
  3. Marguet D, et al. 1999. Immunity 11:231.
  4. Bashuda H, et al. 1997. Transplantation 63:113.
  5. Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed
  6. Milstein O, et al. 2011. Blood 117:1042.
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Azuma T, et al. 2008. Blood. 111:3635. PubMed
  3. Frey BF, et al. 2018. J Immunol. 200:1853. PubMed
  4. Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed
AB_313583 (BioLegend Cat. No. 114502)

Antigen Details

MHC class I

Nucleated cells of the H-2Ld/H-2Db haplotype

Antigen presentation
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
View information about H-2Ld, H-2Db on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08/16/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse H-2Ld/H-2Db

  • PE anti-mouse H-2Ld/H-2Db


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