Purified anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
W19132B (See other available formats)
Regulatory Status
RUO
Other Names
SignalingLymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
W19132B_PURE_CD150_Antibody_011921
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 APC and purified anti-mouse CD150 (SLAM) (clone W19132B) (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • W19132B_PURE_CD150_Antibody_011921
    C57BL/6 mouse splenocytes were stained with anti-mouse CD19 APC and purified anti-mouse CD150 (SLAM) (clone W19132B) (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
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162602 100 µg 104,00€
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
RBL1 transfectant (1-287a.a)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894667 (BioLegend Cat. No. 162602)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Antigen References

1. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
2. Gordiienko I, et al. 2019. Clin Immunol. 204:14-22
3. Kiel M, et al. 2005. Cell. 121(7):1109-21

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/18/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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