Purified anti-METTL14 Antibody

Pricing & Availability
Clone
W18219A (See other available formats)
Other Names
Methyltransferase Like 14, N6-Adenosine-Methyltransferase Non-Catalytic Subunit, HMETTL14, KIAA1627
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18219A_PURE_METTL14_Antibody_1_072519_updated
Whole cell lysates (15 µg) from the listed cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution from 0.5 mg/mL) of purified anti-METTL14 antibody, clone W181219, overnight at 4°C. The following day, protein was chemiluminescently detected via a 1 hour room temp. incubation with HRP-conjugated Goat anti-rat (1:3000 dilution; for in-house target) and HRP-conjugated Goat anti-rabbit (1:3000 dilution) followed by imaging.
  • W18219A_PURE_METTL14_Antibody_1_072519_updated
    Whole cell lysates (15 µg) from the listed cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution from 0.5 mg/mL) of purified anti-METTL14 antibody, clone W181219, overnight at 4°C. The following day, protein was chemiluminescently detected via a 1 hour room temp. incubation with HRP-conjugated Goat anti-rat (1:3000 dilution; for in-house target) and HRP-conjugated Goat anti-rabbit (1:3000 dilution) followed by imaging.
  • W18219A_PURE_METTL14_Antibody_2_072519_updated
    Whole Cell Lysates (250 µg) were prepared from MOLT4 cells and then immunoprecipitated overnight with 2.5 µg of purified rat IgG2a isotype control antibody or purified METTL14 antibody (clone W181219). The resulting fractions and 5% whole cell lysate input were resolved by 4-12% Bis-Tris Gel Electrophoresis and probed with an anti-rat secondary antibody against the target.
Cat # Size Price Quantity Avail. Save
669601 25 µg 68€
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669602 100 µg 172€
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Description

METTL14 forms a heterodimer with METTL3 called the m6A-methyltransferase complex (MTC) to methylate adenosine residues at the N6 position of eukaryotic mRNAs. MTC-induced methylation takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs. In the MTC complex, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate. Once methylated, the mRNAs are recognized by distinct proteins known as “readers” that will affect their stability, splicing, and/or translation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial-length recombinant human METTL14 protein between 1-300 a.a.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While this clone is able to immunoprecipitate METTL14 from whole cell extracts, the resulting fraction was not significantly enriched in METTL14; therefore, this clone may not be suitable for detecting transient or low-abundance complexes.

RRID
AB_2814512 (BioLegend Cat. No. 669601)
AB_2814513 (BioLegend Cat. No. 669602)

Antigen Details

Structure
METTL14 is a 456 amino acid protein with a molecular weight of 65 kD.
Distribution

Localized to the mitochondria; ubiquitously expressed.

Function
Methylation; mRNA splicing; mRNA translation
Interaction
METTL3
Biology Area
Protein Synthesis
Antigen References
  1. Liu J, et al. 2014. Nat. Chem. Biol. 10.1038/nchembio.1432.
  2. Nagase T, et al. 2000. DNA Res. 7:273-81.
  3. Yue Y, et al. 2018. Cell Discov. 4:10.
  4. Schöller E, et al. 2018. RNA. 10.1261/rna.064063.117.
  5. Wang X, et al. 2016. Nature. 10.1038/nature18298.
Gene ID
57721 View all products for this Gene ID
UniProt
View information about METTL14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/19/2019

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